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Literature summary extracted from

  • Okamoto, Y.; Morishita, J.; Tsuboi, K.; Tonai, T.; Ueda, N.
    Molecular characterization of a phospholipase D generating anandamide and its congeners (2003), J. Biol. Chem., 279, 5298-5305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.54 overexpression in COS-7 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.54 0.0028
-
 N-arachidonoyl-1,2-dioleoylphosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus
3.1.4.54 0.0029
-
 N-oleoyl-1,2-dioleoylphosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus
3.1.4.54 0.0033
-
 N-palmitoyl-1-palmitoyl-2-linoleoylphosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus
3.1.4.54 0.0034
-
 N-stearoyl-1,2-dioleoylphosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus
3.1.4.54 0.004
-
 N-palmitoyl-1-palmitoyl-2-lysophosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus
3.1.4.54 0.004
-
 N-arachidonoyl-1-oleoyl-2-lysophosphatidylethanolamine pH 7.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.54 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.54 Zinc member of the zinc metallohydrolase family of the beta-lactamase fold Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.54 Rattus norvegicus Q769K2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.54
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.54 brain
-
 Rattus norvegicus
-
3.1.4.54 heart
-
 Rattus norvegicus
-
3.1.4.54 kidney
-
 Rattus norvegicus
-
3.1.4.54 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.54 0.0979
-
 substrate: N-palmitoyl-1-palmitoyl-2-linoleoyl-phosphatidylethanolamine, pH 7.5, 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.54 additional information the enzyme is inactive with phosphatidylcholine and phosphatidylethanolamine. No transphosphatidation Rattus norvegicus ?
-
 ?
3.1.4.54 N-arachidonoyl-1,2-dioleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-arachidonoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
3.1.4.54 N-arachidonoyl-1-oleoyl-2-lysophosphatidylethanolamine + H2O
-
 Rattus norvegicus N-arachidonoylethanolamine + 1-oleoyl-2-lyso-sn-glycerol 3-phosphate
-
 ?
3.1.4.54 N-oleoyl-1,2-dioleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-oleoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
3.1.4.54 N-palmitoyl-1-palmitoyl-2-linoleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-palmitoylethanolamine + 1-palmitoyl-2-linoleoyl-sn-glycerol 3-phosphate
-
 ?
3.1.4.54 N-palmitoyl-1-palmitoyl-2-lysophosphatidylethanolamine + H2O
-
 Rattus norvegicus N-palmitoylethanolamine + 1-palmitoyl-2-lyso-sn-glycerol 3-phosphate
-
 ?
3.1.4.54 N-stearoyl-1,2-dioleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-stearoylethanolamine + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.54 NAPE-PLD
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.54 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.54 7.5
-
 assay at Rattus norvegicus