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Literature summary extracted from
- Salicylate biosynthesis in Pseudomonas aeruginosa. Purification and characterization of PchB, a novel bifunctional enzyme displaying isochorismate pyruvate-lyase and chorismate mutase activities (2002), J. Biol. Chem., 277, 21768-21775.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.99.21 | I88T | no isochorismate pyruvate lyase activity, retains chorismate mutase activity | Pseudomonas aeruginosa |
| 4.2.99.21 | additional information | a CM-deficient Escherichia coli mutant, which is auxotrophic for phenylalanine and tyrosine, is functionally complemented by the cloned pchB gene for growth in minimal medium | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.21 | additional information | not inhibitory at 1 mM: EDTA, EGTA, or o-phenanthroline. No substrate inhibition up to 1.2 mM | Pseudomonas aeruginosa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.99.21 | 0.0125 | - |
isochorismate | pH 7.0, 37°C | Pseudomonas aeruginosa |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.21 | additional information | no metal cofactor requirement | Pseudomonas aeruginosa |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.21 | 11500 | - |
2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
| 4.2.99.21 | 14000 | - |
2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
| 4.2.99.21 | 30000 | - |
PAGE | Pseudomonas aeruginosa |
| 4.2.99.21 | 31000 | 34000 | gel filtration | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.99.21 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.99.21 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.21 | 12.89 | - |
pH 7.0, 37°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.21 | isochorismate | - |
Pseudomonas aeruginosa | salicylate + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.99.21 | dimer | 2 * 14000, SDS-PAGE, 2 * 11500, calculated | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.99.21 | 1.76 | - |
isochorismate | pH 7.0, 37°C | Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.21 | 6.8 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.21 | additional information | no cofactor required | Pseudomonas aeruginosa |
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