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Literature summary extracted from

  • Someya, Y.; Takeda, N.
    Insights into the enzyme-substrate interaction in the norovirus 3C-like protease (2009), J. Biochem., 146, 509-521.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.66 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells Chiba virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.66 E54L the E54L mutant protease is a substrate-specificity mutant and requires large hydrophobic amino acid residues at both P4 and P2 positions of the substrate, it cleaves the sequence 133LSFE/AP between the 3B and 3C regions of norovirus polyprotein, but does not cleaves the sequence 198ATSE/GK between the 3A and 3B Chiba virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.66 L-Leu-Gly-Gly
-
 Chiba virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.66 norovirus polyprotein + H2O Chiba virus
-
 hydrolyzed norovirus polyprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.66 Chiba virus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.66 GSTrap FF column chromatography Chiba virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.66 norovirus polyprotein + H2O
-
 Chiba virus hydrolyzed norovirus polyprotein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.66 3C-like protease
-
 Chiba virus
3.4.22.66 3CP
-
 Chiba virus