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Literature summary extracted from

  • Donat, S.; Streker, K.; Schirmeister, T.; Rakette, S.; Stehle, T.; Liebeke, M.; Lalk, M.; Ohlsen, K.
    Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus (2009), J. Bacteriol., 191, 4056-4069.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 Mn2+ 10 mM Mn2+ inhibits phosphorylation of myelin basic protein by PknB Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ maximal kinase activity is detected in the presence of up to 5-10 mM Mg2+ Staphylococcus aureus
2.7.11.1 Mn2+ preferred cofactor, maximal kinase activity is detected in the presence of up to 5 mM Mn2+ Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 75000
-
 SDS-PAGE Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Staphylococcus aureus
-
-
-
2.7.11.1 Staphylococcus aureus 8325
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 Ni-TED column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + adenylosuccinate synthase PknB specifically phosphorylates adenylosuccinate synthase, phosphorylated adenylosuccinate synthase shows a 1.8fold decrease in enzymatic activity compared to unphosphorylated enzyme Staphylococcus aureus ADP + phosphorylated adenylosuccinate synthase
-
 ?
2.7.11.1 ATP + adenylosuccinate synthase PknB specifically phosphorylates adenylosuccinate synthase, phosphorylated adenylosuccinate synthase shows a 1.8fold decrease in enzymatic activity compared to unphosphorylated enzyme Staphylococcus aureus 8325 ADP + phosphorylated adenylosuccinate synthase
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Staphylococcus aureus ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Staphylococcus aureus 8325 ADP + phosphorylated myelin basic protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 PknB
-
 Staphylococcus aureus
2.7.11.1 StpK
-
 Staphylococcus aureus

General Information

EC Number General Information Comment Organism
2.7.11.1 malfunction a pknB deletion strain showed 32-fold-higher sensitivity to tunicamycin Staphylococcus aureus