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Literature summary extracted from

  • Mueller, J.; Nillius, D.; Hehl, A.; Hemphill, A.; Mueller, N.
    Stable expression of Escherichia coli beta-glucuronidase A (GusA) in Giardia lamblia: application to high-throughput drug susceptibility testing (2009), J. Antimicrob. Chemother., 64, 1187-1191.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.31 drug development stable expression of GusA in Gardia lamblia strain WB C6 for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview Escherichia coli
3.2.1.31 molecular biology stable expression of GusA in Gardia lamblia strain WB C6 for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.31 recombinant expression of His-tagged GusA in Escherichia coli strain TOP10 and BL21, stable expression of GusA in Gardia lamblia strain WB C6 trophozoites, under control of the promoter from the glutamate dehydrogenase, gdh, gene, for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 4-nitrophenyl D-glucuronide + H2O
-
 Escherichia coli 4-nitrophenol + D-glucuronate
-
 ?
3.2.1.31 5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O
-
 Escherichia coli 5-bromo-4-chloro-1H-indol-3-ol + beta-D-glucuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 beta-glucuronidase A
-
 Escherichia coli
3.2.1.31 GusA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.31 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 7
-
 assay at Escherichia coli