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Literature summary extracted from

  • Hazra, A.; Chatterjee, A.; Begley, T.P.
    Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction (2009), J. Am. Chem. Soc., 131, 3225-3229.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.10 additional information overexpression of His-tagged proteins ThiF, NifS, ThiO, and ThiSG Escherichia coli BL21and incubation with L-cysteine in the presence of dithiothreitol and ATP to form ThiS-COSH. This is then added to 1-deoxy-D-xylulose 5-phosphate and glycine in the presence of ThiO and ThiG to produce the product of the thiazole synthase-catalyzed reaction. Reaction product is a dearomatized thiazole tautomer Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.10 Bacillus subtilis O31618
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.10 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
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 Bacillus subtilis 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O product is a dearomatized thiazole tautomer, not thiazole phosphate ?