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Literature summary extracted from

  • Hsiao, Y.M.; Liu, Y.F.; Fang, M.C.; Tseng, Y.H.
    Transcriptional regulation and molecular characterization of the manA gene encoding the biofilm dispersing enzyme mannan endo-1,4-beta-mannosidase in Xanthomonas campestris (2010), J. Agric. Food Chem., 58, 1653-1663.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 2-mercaptoethanol 1 mMm, 121% of initial activity Xanthomonas campestris
3.2.1.78 Triton X-100 1%, 123% of initial activity Xanthomonas campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.78 additional information mutant MG17 is catalytically inactive, mutant protein is present at wild-type molecular mass Xanthomonas campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 1 mM, 18% residual activity Xanthomonas campestris
3.2.1.78 Al3+ 1 mM, 31% residual activity Xanthomonas campestris
3.2.1.78 Cu2+ 1 mM, 2% residual activity Xanthomonas campestris
3.2.1.78 Fe3+ 1 mM, 37% residual activity Xanthomonas campestris
3.2.1.78 Hg2+ 1 mM, 1% residual activity Xanthomonas campestris
3.2.1.78 Zn2+ 1 mM, 16% residual activity Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Ca2+ 1 mM, 119% of initial activity Xanthomonas campestris
3.2.1.78 Mg2+ 1 mM, 118% of initial activity Xanthomonas campestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 33000
-
 x * 33000, SDS-PAGE and calculated Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Xanthomonas campestris Q8P9S0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 recombinant enzyme Xanthomonas campestris

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 33000, SDS-PAGE and calculated Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 37
-
-
 Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 7
-
-
 Xanthomonas campestris

Expression

EC Number Organism Comment Expression
3.2.1.78 Xanthomonas campestris isoform manA transcription is positively regulated by Clp and RpfF and induced by locust bean gum up