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Literature summary extracted from

  • Xie, C.; Zhang, Y.; Wang, H.H.; Matsumoto, A.; Nakamura, A.; Ishikawa, R.; Yoshiyama, S.; Hayakawa, K.; Kohama, K.; Gao, Y.
    Calcium regulation of non-kinase and kinase activities of recombinant myosin light-chain kinase and its mutants (2009), IUBMB Life, 61, 1092-1098.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expressed in Escherichia coli BL21(DE3) cells Gallus gallus
2.7.11.18 expressed in Escherichia coli BL21(DE3) cells Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ activates Gallus gallus
2.7.11.18 Ca2+ activates Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Bos taurus Q28824
-
-
2.7.11.18 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 butyl-Toyopearl column chromatography and Sephacryl S-200 gel filtration Gallus gallus
2.7.11.18 calmodulin-Sepharose column chromatography, DEAE 650M Toyopearl column chromatography, and Superose 6 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 gizzard
-
 Gallus gallus
-
2.7.11.18 stomach
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain 20 kDa regulatory light chain of smooth muscle Gallus gallus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain 20 kDa regulatory light chain of smooth muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 additional information the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities Gallus gallus ?
-
 ?
2.7.11.18 additional information the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Gallus gallus
2.7.11.18 MLCK
-
 Bos taurus
2.7.11.18 myosin light-chain kinase
-
 Gallus gallus
2.7.11.18 myosin light-chain kinase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Gallus gallus
2.7.11.18 ATP
-
 Bos taurus
2.7.11.18 Calmodulin
-
 Gallus gallus
2.7.11.18 Calmodulin
-
 Bos taurus