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Literature summary extracted from

  • Gomes, E.; Leite, R.S.; da Silva, R.; Silva, D.
    Purification of an exopolygalacturonase from Penicillium viridicatum RFC3 produced in submerged fermentation (2009), Int. J. Microbiol., 2009, 631942.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.67 Ca2+ enhances the stability of enzyme and its activity by 30% Penicillium viridicatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ag+ 7% inhibition at 10 mM, 13% inhibition at 2 mM Penicillium viridicatum
3.2.1.67 Al3+ 33% inhibition at 2 mM, no inhibition at 10 mM Penicillium viridicatum
3.2.1.67 Ba2+ 10% inhibition at 2-5 mM Penicillium viridicatum
3.2.1.67 Co2+ 7% inhibition at 10 mM, 7% activation at 2 mM Penicillium viridicatum
3.2.1.67 Cu2+ 67% inhibition at 2 mM Penicillium viridicatum
3.2.1.67 Fe3+ 85% inhibition at 5 mM Penicillium viridicatum
3.2.1.67 Hg2+ complete inhibition at 5 mM Penicillium viridicatum
3.2.1.67 K+ 37% inhibition at 2 mM, 16% inhibition at 10 mM Penicillium viridicatum
3.2.1.67 Mg2+ 73% inhibition at 2 mM Penicillium viridicatum
3.2.1.67 Mn2+ 10% inhibition at 10 mM, 10% activation at 2 mM Penicillium viridicatum
3.2.1.67 Ni2+ 37% inhibition at 2 mM, 19% inhibition at 10 mM Penicillium viridicatum
3.2.1.67 Zn2+ complete inhibition at 5 mM Penicillium viridicatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information kinetics in presence and absence of Ca2+, overview Penicillium viridicatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Ca2+ Ca2+ enhances the stability of enzyme, and its activity by 10% at 2 mM and 10 mM, and by 30% at 5 mM Penicillium viridicatum
3.2.1.67 Co2+ 7% inhibition at 10 mM, 7% activation at 2 mM Penicillium viridicatum
3.2.1.67 Mn2+ 10% inhibition at 10 mM, 10% activation at 2 mM Penicillium viridicatum
3.2.1.67 Na+ 23% activation at 2 mM, no effect at 10 mM Penicillium viridicatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 92000
-
 x * 92000, SDS-PAGE Penicillium viridicatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 pectin + H2O Penicillium viridicatum the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O Penicillium viridicatum RFC3 the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification galacturonic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Penicillium viridicatum
-
 isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil
-
3.2.1.67 Penicillium viridicatum RFC3
-
 isolated from decaying vegetables in Sao Jose do Rio Preto, SP, Brazil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 native enzyme 37.7fold from submerged fermentation to homogeneity Penicillium viridicatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 submerged culture
-
 Penicillium viridicatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 346.4
-
 purified native enzyme Penicillium viridicatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 apple pectin + H2O the activity on citrus pectin is appreciably higher than that on apple pectin Penicillium viridicatum galacturonic acid + ?
-
 ?
3.2.1.67 apple pectin + H2O the activity on citrus pectin is appreciably higher than that on apple pectin Penicillium viridicatum RFC3 galacturonic acid + ?
-
 ?
3.2.1.67 citric pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification Penicillium viridicatum galacturonic acid + ?
-
 ?
3.2.1.67 citric pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification, the substrate shows 92% esterification Penicillium viridicatum RFC3 galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification Penicillium viridicatum galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O the enzyme releases galacturonic acid by hydrolysis of pectin with a high degree of esterification Penicillium viridicatum RFC3 galacturonic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 92000, SDS-PAGE Penicillium viridicatum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 Exo-PG
-
 Penicillium viridicatum
3.2.1.67 exo-polygalacturonase
-
 Penicillium viridicatum
3.2.1.67 exopolygalacturonase
-
 Penicillium viridicatum
3.2.1.67 PG III
-
 Penicillium viridicatum
3.2.1.67 polymethylgalacturonase
-
 Penicillium viridicatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50 55
-
 Penicillium viridicatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 35 70 profile, overview Penicillium viridicatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 5 55 purified enzyme, absence of substrate, 1 h, 10 mM Ca2+, 80-100% activity remaining Penicillium viridicatum
3.2.1.67 5 35 purified enzyme, absence of substrate, 1 h, 85-100% activity remaining Penicillium viridicatum
3.2.1.67 60
-
 purified enzyme, loss of 50% activity in absence of Ca2+ after 25 min, in presence of 10 mM Ca2+ after 37 min Penicillium viridicatum
3.2.1.67 70
-
 purified enzyme, absence of substrate, 1 h, 45% activity remaining, 70% in presence of 10 mM Ca2+ Penicillium viridicatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 5
-
-
 Penicillium viridicatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 4 8 50% of maximal activity at pH 8.0, profile, overview Penicillium viridicatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 4 5 in presence of Ca2+, stable Penicillium viridicatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.67 Penicillium viridicatum isoelectric focusing
-
 5.4