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Literature summary extracted from

  • Dave, S.; Dkhar, H.K.; Singh, M.P.; Gupta, G.; Chandra, V.; Mahajan, S.; Gupta, P.
    Hexafluoroisopropanol-induced helix-sheet transition of stem bromelain: correlation to function (2010), Int. J. Biochem. Cell Biol., 42, 938-947.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.32 medicine the nonnative form of SBM shows only a fraction of the native protein's enzymatic activity but is more effective at inhibiting cell growth and displays better antitumorigenic properties than native SBM Ananas comosus

General Stability

EC Number General Stability Organism
3.4.22.32 treatment with 10-30% (v/v) 1,1,1,3,3,3-hexafluoroisopropanol induces the partially folded intermediate to adopt much of the native protein’s secondary structure, but only a rudimentary tertiary structure, characteristic of the molten globule state. Addition of slightly higher concentrations of 1,1,1,3,3,3-hexafluoroisopropanol causes transformation from an alpha-helix to a beta-sheet and induces formation of a compact nonnative structure Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
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-
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Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Sbm
-
 Ananas comosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.32 63
-
 thermal unfolding of native SBM occurs at 63°C Ananas comosus