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Literature summary extracted from

  • Sarkar, S.K.; Chowdhury, C.; Ghosh, A.S.
    Deletion of penicillin-binding protein 5 (PBP5) sensitises Escherichia coli cells to beta-lactam agents (2010), Int. J. Antimicrob. Agents, 35, 244-249.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.16.4 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.4 amoxicillin
-
 Escherichia coli
3.4.16.4 ampicillin
-
 Escherichia coli
3.4.16.4 cefaclor
-
 Escherichia coli
3.4.16.4 cefadroxil
-
 Escherichia coli
3.4.16.4 cefalexin
-
 Escherichia coli
3.4.16.4 cefalothin
-
 Escherichia coli
3.4.16.4 penicillin G
-
 Escherichia coli
3.4.16.4 piperacillin
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.4 Escherichia coli
-
 strains K-12 and 2443
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.4 ampicillin-affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.4 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Escherichia coli Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.4 DD-Carboxypeptidase
-
 Escherichia coli
3.4.16.4 DD-CPase
-
 Escherichia coli
3.4.16.4 PBP5
-
 Escherichia coli
3.4.16.4 penicillin binding protein 5
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
3.4.16.4 malfunction loss of penicillin-binding protein 5 enhances beta-lactam susceptibility, the observed susceptibilities for ampicillin, piperacillin, amoxicillin, penicillin G, cefadroxil and cefalexin are enhanced by 4fold and for cefalothin and cefaclor the susceptibilities are at least 8fold higher in the mutants Escherichia coli