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Literature summary extracted from

  • Chen, J.; Aimanova, K.G.; Pan, S.; Gill, S.S.
    Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin (2009), Insect Biochem. Mol. Biol., 39, 688-696.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 expressed in Escherichia coli DH10B cells (binds Cry11Aa toxin with high affinity) and in Sf21 cells (enzymatically active, has a GPI-anchor but does not bind Cry11Aa toxin) Aedes aegypti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 membrane
-
 Aedes aegypti 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 110000
-
 in Sf12 cells infected with recombinant baculovirus containing full-length APN,1 the anti-APN1 antibody detects a 110000 Da band, gel filtration Aedes aegypti
3.4.11.2 112000
-
 calculated from amino acid sequence Aedes aegypti
3.4.11.2 140000
-
 native enzyme, gel filtration Aedes aegypti

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Aedes aegypti Q16L33
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.2 additional information the native but Sf21 cell-expressed APN1 contains N-acetylglucosamine moieties Aedes aegypti

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 Ni-NTA column chromatography and Sephadex G25 gel filtration Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 epithelial cell the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-
3.4.11.2 larva the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-
3.4.11.2 midgut the protein localizes to the apical side of posterior midgut epithelial cells of larva Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Aedes aegypti L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O
-
 Aedes aegypti L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 L-Val-4-nitroanilide + H2O
-
 Aedes aegypti L-Val + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase N
-
 Aedes aegypti
3.4.11.2 APN1
-
 Aedes aegypti

General Information

EC Number General Information Comment Organism
3.4.11.2 physiological function aminopeptidase N is a putative receptor of Bacillus thuringiensis Cry11A toxin Aedes aegypti