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Literature summary extracted from

  • Nakamura, N.; Stalnaker, S.H.; Lyalin, D.; Lavrova, O.; Wells, L.; Panin, V.M.
    Drosophila Dystroglycan is a target of O-mannosyltransferase activity of two protein O-mannosyltransferases, Rotated Abdomen and Twisted (2010), Glycobiology, 20, 381-394.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.109 medicine using Drosophila as model for investigating the mechanisms of human congenital muscular dystrophies (dystroglycanopathies) resulting from the abnormal glycosylation of alpha-dystroglycan Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.109 additional information mutants lacking or overexpressing one of the two or both O-mannosyltransferases Rotated Abdomen and Twisted show that both are required for in vivo production of high molecular mass dystroglycan, overexpression of only one isoform does not increase its amount over wild-type, lack of either one of the isoforms or both isoforms lacking double mutant do not produce the high molecular mass dystroglycan Drosophila melanogaster
2.4.1.109 yes mutants lacking or overexpressing one of the two or both O-mannosyltransferases Rotated Abdomen and Twisted show that both are required for in vivo production of high molecular mass dystroglycan, overexpression of only one isoform does not increase its amount over wild-type, lack of either one of the isoforms or both isoforms lacking double mutant do not produce the high molecular mass dystroglycan Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 microsome
-
 Drosophila melanogaster
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + alpha-dystroglycan Drosophila melanogaster in vitro asssay with both enzyme isoforms (RT and TW), 20 mM Tris, pH 8.0, mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, sugar donor is tritium-labeled, alpha-dystroglycan isoform C contains mucin-type domain that is target of O-mannose modifications in mammals, high grade of O-mannosylation, A isoform without noticeable O-mannosylation, rabbit alpha-dystroglycan as positive control shows lower O-mannosylation dolichyl phosphate + O-D-mannosyl-[alpha-dystroglycan]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.109 larva
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + alpha-dystroglycan in vitro asssay with both enzyme isoforms (RT and TW), 20 mM Tris, pH 8.0, mercaptoethanol, EDTA, n-octyl-beta-D-thioglucoside, sugar donor is tritium-labeled, alpha-dystroglycan isoform C contains mucin-type domain that is target of O-mannose modifications in mammals, high grade of O-mannosylation, A isoform without noticeable O-mannosylation, rabbit alpha-dystroglycan as positive control shows lower O-mannosylation Drosophila melanogaster dolichyl phosphate + O-D-mannosyl-[alpha-dystroglycan]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 Drosophila O-mannosyltransferase Rotated Abdomen
-
 Drosophila melanogaster
2.4.1.109 Drosophila O-mannosyltransferase Twisted
-
 Drosophila melanogaster
2.4.1.109 O-mannosyltransferase
-
 Drosophila melanogaster
2.4.1.109 RT
-
 Drosophila melanogaster
2.4.1.109 TW
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.4.1.109 malfunction defects in O-mannosylation are associated with muscular dystrophies (dystroglycanopathies) Drosophila melanogaster
2.4.1.109 physiological function O-mannosylation can modulate the ligand-binding activity of alpha-dystroglycan Drosophila melanogaster