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Literature summary extracted from

  • Watanabe, T.; Totani, K.; Matsuo, I.; Maruyama, J.; Kitamoto, K.; Ito, Y.
    Genetic analysis of glucosidase II beta-subunit in trimming of high-mannose-type glycans (2009), Glycobiology, 19, 834-840.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.207 genes Aogls2alpha and/or Aogls2beta encdoing the alpha- und beta-subunit, cloning and expression in Escherichia coli strain DH5alpha Aspergillus oryzae

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.207 additional information construction of strains of gene disruptants lacking either the glucosidase II alpha- or beta-subunit. The mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and Glc1Man9GlcNAc2, but activity can be restored by adding the beta-subunit fraction, overview Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.207 deoxynojirimycin an alpha-glucosidase-specific inhibitor Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.207 endoplasmic reticulum membrane
-
 Aspergillus oryzae 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.207 additional information Aspergillus oryzae glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum ?
-
 ?
3.2.1.207 additional information Aspergillus oryzae RIB 40 glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Aspergillus oryzae
-
-
-
3.2.1.207 Aspergillus oryzae RIB 40
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Aspergillus oryzae RIB 40 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G1M9-MTX Aspergillus oryzae GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G1M9-MTX Aspergillus oryzae RIB 40 GlcMan9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G2M9-MTX Aspergillus oryzae Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 GlcMan9GlcNAc2 + H2O synthetic methotrexate-coupled glycan substrate, G2M9-MTX Aspergillus oryzae RIB 40 Man9GlcNAc2 + D-glucopyranose
-
 ?
3.2.1.207 additional information glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum Aspergillus oryzae ?
-
 ?
3.2.1.207 additional information the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 Aspergillus oryzae ?
-
 ?
3.2.1.207 additional information glucosidase II is a glycoprotein-processing enzyme that successively cleaves two alpha1,3-linked glucose residues from N-linked oligosaccharides in the endoplasmic reticulum Aspergillus oryzae RIB 40 ?
-
 ?
3.2.1.207 additional information the mutant lacking the beta-subunit is inactive with both Glc2Man9GlcNAc2 and GlcMan9GlcNAc2 Aspergillus oryzae RIB 40 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 heterodimer the enzyme is a heterodimer whose alpha-subunit contains a glycosidase active site, while the beta-subunit confers the substrate specificity toward di- and monoglucosylated glycans on the glucose-trimming activity of the alpha-subunit Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.207 glucosidase II
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.207 37
-
 assay at Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.207 7.4
-
 assay at Aspergillus oryzae