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Literature summary extracted from

  • Ashida, H.; Miyake, A.; Kiyohara, M.; Wada, J.; Yoshida, E.; Kumagai, H.; Katayama, T.; Yamamoto, K.
    Two distinct alpha-L-fucosidases from Bifidobacterium bifidum are essential for the utilization of fucosylated milk oligosaccharides and glycoconjugates (2009), Glycobiology, 19, 1010-1017.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.63 gene afcA, expression in Escherichia coli and in enzyme-deficient Bifidobacterium longum strain 105-A which renders the transformant able to utilize 2'-fucosyllactose as the sole carbon source Bifidobacterium bifidum
3.2.1.111 expression in Escherichia coli. Introduction of afcB gene into the Bacterioides longum 105-A strain, which has no intrinsic alpha-L-fucosidase. The transformant is able to utilize 3-fucosyllactose and lacto-N-fucopentaose II as the sole carbon source Bifidobacterium bifidum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.111 additional information introduction of afcB gene into the Bacterioides longum 105-A strain, which has no intrinsic alpha-L-fucosidase. The transformant is able to utilize 3-fucosyllactose and lacto-N-fucopentaose II as the sole carbon source Bifidobacterium bifidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.111 Co2+ 40% residual activity Bifidobacterium bifidum
3.2.1.111 Cu2+ complete inactiviation Bifidobacterium bifidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.111 160000
-
 2 * 160000, SDS-PAGE Bifidobacterium bifidum
3.2.1.111 350000
-
 gel filtration Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.63 2'-fucosyllactose + H2O Bifidobacterium bifidum
-
 L-fucose + lactose
-
 ?
3.2.1.111 additional information Bifidobacterium bifidum Bifidobacteria are predominant bacteria present in the intestines of breast-fed infants and offer important health benefits for the host. alpha-L-Fucosidase AfcB play essential roles in degrading alpha1,3/4-fucosylated milk oligosaccharides, respectively, and also glycoconjugates, in the gastrointestinal tracts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.63 Bifidobacterium bifidum
-
 strain JCM1254, gene afcA
-
3.2.1.63 no activity in Bifidobacterium longum
-
-
-
3.2.1.111 Bifidobacterium bifidum
-
 JCM1254
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.63 2'-fucosyllactose + H2O
-
 Bifidobacterium bifidum L-fucose + lactose
-
 ?
3.2.1.111 L-Fuc-alpha-(1->2)-D-Gal-beta-(1->3)-[L-Fuc-alpha-(1->4)]-GlcNAc + H2O i.e. Lewis b tetrasaccharide, 14% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose + L-Fuc-alpha-(1->2)-D-Gal-beta-(1->3)-D-GlcNAc
-
 ?
3.2.1.111 L-Fuc-alpha-(1->2)-D-Gal-beta-(1->4)-[L-Fuc-alpha-(1->3)]-D-GlcNAc + H2O i.e. Lewis y tetrasaccharide, 128% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose + L-Fuc-alpha-(1->2)-D-Gal-beta-(1->4)-D-GlcNAc
-
 ?
3.2.1.111 L-Fuc-alpha-(1->3)-[D-Gal-beta-(1->4)]-D-Glc + H2O i.e. 3-fucosyllactose Bifidobacterium bifidum L-fucose + D-Gal-beta-(1->4)-D-Glc
-
 ?
3.2.1.111 L-Fuc-alpha-(1->3)-[D-Gal-beta-(1->4)]-D-GlcNAc + H2O i.e. Lewis a trisaccharide, 160% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose + D-Gal-beta-(1->4)-D-GlcNAc
-
 ?
3.2.1.111 L-Fuc-alpha-(1->3)-[D-Gal-beta-(1->4)]-D-GlcNAc-beta-(1->3)-D-Gal-beta-(1->4)-D-Glc + H2O i.e. lacto-N-fucopentaose III, 159% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose + D-Gal-beta-(1->4)-D-GlcNAc-beta-(1->3)-D-Gal-beta-(1->4)-D-Glc
-
 ?
3.2.1.111 L-Fuc-alpha-(1->4)-D-Gal-beta-(1->3)-D-GlcNAc-beta-(1->3)-D-Gal-beta-(1->4)-D-Glc + H2O i.e. lacto-N-fucopentaose II, 186% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose +
-
 ?
3.2.1.111 L-Fuc-alpha-(1->4)-[D-Gal-beta-(1->3)]-D-GlcNAc + H2O i.e. Lewis a trisaccharide, 166% of the activity with 3-fucosyllactose Bifidobacterium bifidum L-fucose + D-Gal-beta-(1->3)-D-GlcNAc
-
 ?
3.2.1.111 additional information Bifidobacteria are predominant bacteria present in the intestines of breast-fed infants and offer important health benefits for the host. alpha-L-Fucosidase AfcB play essential roles in degrading alpha1,3/4-fucosylated milk oligosaccharides, respectively, and also glycoconjugates, in the gastrointestinal tracts Bifidobacterium bifidum ?
-
 ?
3.2.1.111 additional information enzyme specifically releases alpha-1,3- and alpha-1,4-linked fucosyl residues from 3-fucosyllactose, various Lewis blood group substances, and lacto-N-fucopentaose II and III. The enzyme does not act on glycoconjugates containing alpha-1,2-fucosyl residue or on synthetic alpha-fucoside, i.e. p-nitrophenyl-alpha-L-fucoside Bifidobacterium bifidum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.111 dimer 2 * 160000, SDS-PAGE Bifidobacterium bifidum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.63 AfcA
-
 Bifidobacterium bifidum
3.2.1.63 More AfcA, an inverting glycosidase, belongs to the glycoside hydrolase family GH95 Bifidobacterium bifidum
3.2.1.111 AfcB
-
 Bifidobacterium bifidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.111 45
-
-
 Bifidobacterium bifidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.111 50
-
 stable below, pH 6.0 Bifidobacterium bifidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.111 5.5 6
-
 Bifidobacterium bifidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.111 3.5 8 stable Bifidobacterium bifidum

General Information

EC Number General Information Comment Organism
3.2.1.63 physiological function AfcA plays essential role in degrading alpha1,2-fucosylated milk oligosaccharides and also glycoconjugates, in the gastrointestinal tracts of the host Bifidobacterium bifidum
3.2.1.111 physiological function Bifidobacteria are predominant bacteria present in the intestines of breast-fed infants and offer important health benefits for the host. alpha-L-Fucosidase AfcB play essential roles in degrading alpha1,3/4-fucosylated milk oligosaccharides, respectively, and also glycoconjugates, in the gastrointestinal tracts Bifidobacterium bifidum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.111 0.46
-
 L-Fuc-alpha-(1->3)-[D-Gal-beta-(1->4)]-D-Glc pH 6.0, 37°C Bifidobacterium bifidum