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Literature summary extracted from

  • Kokuho, T.; Yasukochi, Y.; Watanabe, S.; Inumaru, S.
    Molecular cloning and expression profile analysis of a novel beta-D-N-acetylhexosaminidase of domestic silkworm (Bombyx mori) (2010), Genes Cells, 15, 525-536.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 gene and isozyme BmGlcNAcase 1, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method Bombyx mori
3.2.1.52 gene and isozyme BmGlcNAcase 2, DNA and amino acid sequence determnination and analysis, genetic structure, phylogenetic analysis, and sequence comparisons, expression in Trichoplusia ni TN5 cells using the baculovirus tranfection method Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 2-acetamido-1,2-deoxynojirimycin
-
 Bombyx mori
3.2.1.52 additional information no inhibition by 1-deoxynojirimycin and 1,4-deoxy-1,4-imino-D-arabinitol; no inhibition by 2-acetamide-1,2-deoxynojirymycin, 1-deoxynojirimycin, and 1,4-deoxy-1,4-imino-D-arabinitol Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 61500
-
 x * 61500, isozyme BmGlcNAcase 2 Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Bombyx mori P49010 isozyme BmGlcNAcase 1; isozyme BmGlcNAcase 1
-
3.2.1.52 Bombyx mori Q3L6N4 isozyme A or BmGlcNAcase 2; isozyme BmGlcNAcase 2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 BmN cell
-
 Bombyx mori
-
3.2.1.52 embryo
-
 Bombyx mori
-
3.2.1.52 fat body of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 fat body of fifth instar larvae, low expression level of BmGlcNAcase 2 Bombyx mori
-
3.2.1.52 head of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 head of fifth instar larvae, low expression level of BmGlcNAcase 2 Bombyx mori
-
3.2.1.52 hemolymph of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 larva BmGlcNAcase 2, first to fourth instar stage, only in some tissue in the fifth instar larvae stage, overview Bombyx mori
-
3.2.1.52 larva first to fifth instar stage Bombyx mori
-
3.2.1.52 malpighian tubule of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 mid intestine of fifth instar larvae Bombyx mori
-
3.2.1.52 mid intestine of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 additional information no expression of BmGlcNAcase 2 in pupa Bombyx mori
-
3.2.1.52 ovary of fifth instar larvae Bombyx mori
-
3.2.1.52 ovary of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 pupa
-
 Bombyx mori
-
3.2.1.52 silk gland of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 skin of fifth instar larvae, BmGlcNAcase 1 Bombyx mori
-
3.2.1.52 skin of fifth instar larvae, low expression level of BmGlcNAcase 2 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O
-
 Bombyx mori 4-nitrophenol + N-acetylgalactosamine
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
-
 Bombyx mori 4-nitrophenol + N-acetylglucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 61500, isozyme BmGlcNAcase 2 Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 BmGlcNAcase 1
-
 Bombyx mori
3.2.1.52 BmGlcNAcase 2
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 37
-
 assay at Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.5 6.5
-
 Bombyx mori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3.5 7.5 the isozyme shows 70% of maximal activity pH 3.5, 30% at pH 7.5 Bombyx mori
3.2.1.52 4.5 7.5 50% of maximal activity at pH 4.5 and pH 7.5, inactive at pH 3.5 Bombyx mori