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Literature summary extracted from

  • Lin, Y.; Hsiao, Y.; Chiang, B.
    Production of high degree polymerized chitooligosaccharides in a membrane reactor using purified chitosanase from Bacillus cereus (2009), Food Res. Int., 42, 1355-1361.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus cereus
-
-
-
3.2.1.132 Bacillus cereus NTU-FC-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 native enzyme 281fold by three steps of anion exchange chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan hexasaccharide + H2O
-
Bacillus cereus chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
-
?
3.2.1.132 chitosan hexasaccharide + H2O
-
Bacillus cereus NTU-FC-4 chitosan disaccharide + chitosan trisaccharide + chitosan tetrasaccharide
-
?
3.2.1.132 chitosan pentasaccharide + H2O hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 Bacillus cereus chitosan disaccharide + chitosan trisaccharide
-
?
3.2.1.132 chitosan pentasaccharide + H2O hydrolysis of the chitosan oligosaccharide (GlcN)5 to (GlcN)2 and (GlcN)3 Bacillus cereus NTU-FC-4 chitosan disaccharide + chitosan trisaccharide
-
?
3.2.1.132 additional information hydrolysis profiles, overview Bacillus cereus ?
-
?
3.2.1.132 additional information hydrolysis profiles, overview Bacillus cereus NTU-FC-4 ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 45
-
assay at Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5
-
assay at Bacillus cereus