Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vujcic, Z.; Loncar, N.; Dojnov, B.; Milovanovic, A.; Vujcic, M.; Bozic, N.
    Characterisation of leucyl aminopeptidase from Solanum tuberosum tuber (2010), Food Chem., 121, 418-423.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 75% inhibition at 2 mM Solanum tuberosum
3.4.11.1 cysteine 20% inhibition at 2 mM Solanum tuberosum
3.4.11.1 additional information no or poor inhibition by 2 mM of iodoacetic acid, EDTA, EGTA, and citrate Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.00925
-
 L-leucyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
3.4.11.1 0.083
-
 L-methionyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
3.4.11.1 0.099
-
 L-Lysyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum
3.4.11.1 0.36
-
 L-alanyl-4-nitroanilide pH 9.0, 37°C Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ stimulates by 100% Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 48000
-
 2 * 48000, SDS-PAGE Solanum tuberosum
3.4.11.1 90000
-
 gel filtration Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Solanum tuberosum
-
 cv. Kennebec
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 native enzyme 200fold from tubers by gel filtration, anion exchange chromatography, and again gel filtration to homogeneity Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 tuber 1-year-old tubers Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-alanyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-alanine + 4-nitroaniline
-
 ?
3.4.11.1 L-leucyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-leucine + 4-nitroaniline
-
 ?
3.4.11.1 L-lysyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-lysine + 4-nitroaniline
-
 ?
3.4.11.1 L-methionyl-4-nitroanilide + H2O
-
 Solanum tuberosum L-methionine + 4-nitroaniline
-
 ?
3.4.11.1 additional information the enzyme is equally active with the synthetic substrates Solanum tuberosum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 dimer 2 * 48000, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 45
-
-
 Solanum tuberosum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.1 10 60 activity range, profile, overview Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 70
-
 purified enzyme, inactivation Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 9
-
-
 Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 5 10.5 activity range, profile, overview Solanum tuberosum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.1 Solanum tuberosum isoelectric focusing
-
 5.45