Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, X.; Ding, S.
    Molecular characterization of a new acetyl xylan esterase (AXEII) from edible straw mushroom Volvariella volvacea with both de-O-acetylation and de-N-acetylation activity (2009), FEMS Microbiol. Lett., 295, 50-56.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 full-length cDNA or 5'-end DNA fragment subcloned into the pGEM-T vector. Full-length gene encoding the mature esterase fused with a 6-histidine tag attached at the C-terminal and ligated at the EcoR1/Xba1 sites of the Picchia pastoris expression vector pPICZaA to yield the construct pPICZaA-AXEII. Transformed and expressed in Pichia pastoris Volvariella volvacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.72 Co2+ inhibits at higher (10 mM) concentrations Volvariella volvacea
3.1.1.72 Cu2+ 1 mM decreases enzyme activity by 12% Volvariella volvacea
3.1.1.72 EDTA concentrations greater than 1 mM almost eliminate the activity, but simultaneous addition of Co2+ (1 mM) and Mg2+ (1 mM) restores the activity Volvariella volvacea
3.1.1.72 Fe2+ 1 mM decreases enzyme activity by 59% Volvariella volvacea
3.1.1.72 Mn2+ 1 mM decreases enzyme activity by 32% Volvariella volvacea
3.1.1.72 Zn2+ 1 mM decreases enzyme activity by 67% Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 additional information
-
 additional information 1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min Volvariella volvacea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.72 Co2+ is required for catalysis. Enzyme activity increases by 54% on addition of 0.1 mM Volvariella volvacea
3.1.1.72 Mg2+ is required for catalysis. 1-10 mM slightly enhance enzyme activity by 11-12% Volvariella volvacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 28760
-
 calculated from sequence Volvariella volvacea
3.1.1.72 29000
-
 x * 29000, SDS-PAGE Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Volvariella volvacea B8YIE0 V14
-
3.1.1.72 Volvariella volvacea V14 B8YIE0 V14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 AXEII with the 6-histidine tag purified by affinity chromatography using Ni-NTA column Volvariella volvacea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.72 mycelium 4-day-old cultures Volvariella volvacea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 additional information
-
 in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min: 3448 IU/micromol with acetylated xylan as substrate, 830.9 IU/micromol with glycol chitin as substrate, 306.7 IU/micromol with chitosan as substrate, 361.1 IU/micromol with colloidal chitin as substrate Volvariella volvacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 acetylated chitosan + H2O
-
 Volvariella volvacea ? + acetate
-
 ?
3.1.1.72 acetylated chitosan + H2O
-
 Volvariella volvacea V14 ? + acetate
-
 ?
3.1.1.72 acetylated colloidal chitin + H2O
-
 Volvariella volvacea ? + acetate
-
 ?
3.1.1.72 acetylated colloidal chitin + H2O
-
 Volvariella volvacea V14 ? + acetate
-
 ?
3.1.1.72 acetylated glycol chitin + H2O
-
 Volvariella volvacea ? + acetate
-
 ?
3.1.1.72 acetylated glycol chitin + H2O
-
 Volvariella volvacea V14 ? + acetate
-
 ?
3.1.1.72 acetylated xylan + H2O exhibits activity toward acetylated oat spelt xylan Volvariella volvacea xylan + acetate
-
 ?
3.1.1.72 acetylated xylan + H2O exhibits activity toward acetylated oat spelt xylan Volvariella volvacea V14 xylan + acetate
-
 ?
3.1.1.72 additional information exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate Volvariella volvacea ?
-
 ?
3.1.1.72 additional information exhibits activity toward various chitinous substrates, but is totally inactive against 4-methylumbelliferyl acetate, beta-nitrophenyl acetate, xylose tetraacetate, and alpha-naphthyl acetate Volvariella volvacea V14 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 29000, SDS-PAGE Volvariella volvacea

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase
-
 Volvariella volvacea
3.1.1.72 AXEII
-
 Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 60
-
-
 Volvariella volvacea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 50 60 is very stable at 60°C and, after a 6 h incubation at 50°C, over 95% of the activity remains Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 7
-
-
 Volvariella volvacea

Expression

EC Number Organism Comment Expression
3.1.1.72 Volvariella volvacea axeII transcript levels are high when the fungus is grown on oat spelt xylan (highest level), cellobiose, microcrystalline cellulose, carboxymethyl-cellulose, lactose, galactose, and chitin from crab as carbon sources up