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Literature summary extracted from

  • Scherzinger, D.; Scheffer, E.; Bar, C.; Ernst, H.; Al-Babili, S.
    The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids (2010), FEBS J., 277, 4662-4673.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.65 expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.65 0.0041
-
 beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 0.0219
-
 3-hydroxy-beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 0.0294
-
 beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 0.0438
-
 3-hydroxy-beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.75 0.00415
-
 beta-apo-8'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 0.0219
-
 3-hydroxy-beta-apo-8'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 0.0294
-
 beta-apo-10'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 0.0438
-
 3-hydroxy-beta-apo-10'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.65 Mycobacterium tuberculosis P9WPR5
-
-
1.13.11.65 Mycobacterium tuberculosis H37Rv P9WPR5
-
-
1.13.11.75 Mycobacterium tuberculosis P9WPR5
-
-
1.13.11.75 Mycobacterium tuberculosis H37Rv P9WPR5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.65 3-hydroxy-beta-apo-10'-carotenal + O2
-
 Mycobacterium tuberculosis 3-hydroxy-beta-apo-13-carotenone + 3-hydroxy-beta-apo-15-carotenal plus a minor product presumably representing 3-hydroxy-beta-apo-11-carotenal ?
1.13.11.65 3-hydroxy-beta-apo-8'-carotenal + O2
-
 Mycobacterium tuberculosis ?
-
 ?
1.13.11.65 beta-apo-10'-carotenal + O2
-
 Mycobacterium tuberculosis beta-apo-13-carotenone plus minor amounts of beta-apo-15-carotenal ?
1.13.11.65 beta-apo-8'-carotenal + O2
-
 Mycobacterium tuberculosis beta-apo-13-carotenone + retinal
-
 ?
1.13.11.65 beta-carotene + O2
-
 Mycobacterium tuberculosis retinal + beta-apo-14'-carotenal + beta-apo-13-carotenone
-
 ?
1.13.11.65 lutein + O2
-
 Mycobacterium tuberculosis ?
-
 ?
1.13.11.65 additional information enzyme cleaves the central C15-C15' and an excentric double bond at the C13-C14 position, leading to retinal (C20), beta-apo-14'-carotenal (C22) and beta-apo-13-carotenone (C18) from beta-carotene, as well as the corresponding hydroxylated products from zeaxanthin and lutein. Enzyme also cleaves 3,3'-dihydroxy-isorenieratene representing aromatic carotenoids synthesized by other mycobacteria. The preference for each of the cleavage positions is determined by the hydroxylation and the nature of the ionone ring. Incubation of apocarotenoids shorter than beta-apo-10'-carotenal reveals only weak activity, substrates with a shorter chain length are not converted Mycobacterium tuberculosis ?
-
 ?
1.13.11.65 additional information no substrates: resveratrol, cholecalciferol, phylloquinone and alpha-tocopherol Mycobacterium tuberculosis ?
-
 ?
1.13.11.65 zeaxanthin + O2
-
 Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 3,3'-dihydoxyisorenieratene + O2 54% cleavage at C15-C15' double bond, 45% cleavage at the C13-C14 double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 3,3'-dihydoxyisorenieratene + O2 54% cleavage at C15-C15' double bond, 45% cleavage at the C13-C14 double bond Mycobacterium tuberculosis H37Rv ?
-
 ?
1.13.11.75 3-hydroxy-beta-apo-10'-carotenal + O2 3% cleavage at C15-C15' double bond, 97% cleavage at the C13-C14 double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 3-hydroxy-beta-apo-8'-carotenal + O2 95% cleavage at C15-C15' double bond, 5% cleavage at the C13-C14 double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 beta-apo-10'-carotenal + O2 79% cleavage at C13-C14 double bond, 20% cleavage at the C15-C15' double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 beta-apo-10'-carotenal + O2 79% cleavage at C13-C14 double bond, 20% cleavage at the C15-C15' double bond Mycobacterium tuberculosis H37Rv ?
-
 ?
1.13.11.75 beta-apo-8'-carotenal + O2 86% cleavage at C13-C14 double bond, 14% cleavage at the C15-C15' double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 beta-apo-8'-carotenal + O2 86% cleavage at C13-C14 double bond, 14% cleavage at the C15-C15' double bond Mycobacterium tuberculosis H37Rv ?
-
 ?
1.13.11.75 beta-carotene + O2 30% cleavage at C13-C14 double bond, 69% cleavage at the C15-C15' double bond Mycobacterium tuberculosis retinal + beta-apo-14'-carotenal + beta-apo-13-carotenone
-
 ?
1.13.11.75 lutein + O2 54% cleavage at C15-C15' double bond, 45% cleavage at the C13-C14 double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 additional information MtCCO cleaves the central C15-C15' and an excentric double bond at the C13-C14 position. The preference for each of the cleavage positions is determined by the hydroxylation and the nature of the ionone ring Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 additional information MtCCO cleaves the central C15-C15' and an excentric double bond at the C13-C14 position. The preference for each of the cleavage positions is determined by the hydroxylation and the nature of the ionone ring Mycobacterium tuberculosis H37Rv ?
-
 ?
1.13.11.75 zeaxanthin + O2 83% cleavage at C15-C15' double bond, 17% cleavage at the C13-C14 double bond Mycobacterium tuberculosis ?
-
 ?
1.13.11.75 zeaxanthin + O2 83% cleavage at C15-C15' double bond, 17% cleavage at the C13-C14 double bond Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.65 carotenoid cleavage oxygenase
-
 Mycobacterium tuberculosis
1.13.11.65 CCO
-
 Mycobacterium tuberculosis
1.13.11.65 Rv0654
-
 Mycobacterium tuberculosis
1.13.11.65 Rv0654 gene locus Mycobacterium tuberculosis
1.13.11.75 carotenoid cleavage oxygenase
-
 Mycobacterium tuberculosis
1.13.11.75 CCO
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.65 392.7
-
 beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 561.7
-
 beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 764.3
-
 3-hydroxy-beta-apo-10'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.65 1308
-
 3-hydroxy-beta-apo-8'-carotenal pH 7.8, 28°C Mycobacterium tuberculosis
1.13.11.75 392
-
 beta-apo-8'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 561
-
 beta-apo-10'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 764
-
 3-hydroxy-beta-apo-10'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis
1.13.11.75 1307
-
 3-hydroxy-beta-apo-8'-carotenal pH 7.9, 28°C Mycobacterium tuberculosis