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Literature summary extracted from

  • Liu, Y.; Yoshida, M.; Kurakata, Y.; Miyazaki, T.; Igarashi, K.; Samejima, M.; Fukuda, K.; Nishikawa, A.; Tonozuka, T.
    Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by Coprinopsis cinerea (2010), FEBS J., 277, 1532-1542.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression of His-tagged CcCel6C in Escherichia coli strain BL21(DE3) Coprinopsis cinerea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 purified recombinant His-tagged CcCel6C unbound and in complex with 4-nitrophenyl beta-D-cellotrioside and cellobiose, hanging drop vapour diffusion method, mixing of 0.001 ml of 21.5 mg/ml containing protein solution with 0.001 ml of well solution containing 100 mM HEPES/KOH, pH 7.0, 30% PEG 8000, 150 mM magnesium acetate, soaking of crystals in 60 mM 4-nitrophenyl beta-D-cellotrioside for 2 h or 220 mM cellobiose for 5 min in well solution for complex formation, X-ray diffraction structure determination and analysis at 1.6 A, 1.4 A, and 1.2 A resolution, respectively Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Coprinopsis cinerea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged CcCel6C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Coprinopsis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Coprinopsis cinerea ?
-
 ?
3.2.1.4 additional information CcCel6C exhibits high cellobiohydrolase activity Coprinopsis cinerea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 More CcCel6C consists of a distorted seven-stranded beta/alpha barrel and has an enclosed tunnel, that remains wide, structure, overview Coprinopsis cinerea

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CcCel6C
-
 Coprinopsis cinerea
3.2.1.4 cellobiohydrolase
-
 Coprinopsis cinerea
3.2.1.4 More the enzyme belongs to the glycoside hydrolase family 6 Coprinopsis cinerea