Literature summary extracted from

Kovacs, L.; Alexa, A.; Klement, E.; Kokai, E.; Tantos, A.; Gogl, G.; Sperka, T.; Medzihradszky, K.F.; Toezser, J.; Dombradi, V.; Friedrich, P.
Regulation of calpain B from Drosophila melanogaster by phosphorylation (2009), FEBS J., 276, 4959-4972.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.B37	expressed in Escherichia coli BL21(DE3) cells	Drosophila melanogaster

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.22.B37	C314A	inactive	Drosophila melanogaster
3.4.22.B37	S845E	the mutation causes a small but reproducible increase in the activation of calpain B	Drosophila melanogaster
3.4.22.B37	T747E	the mutation causes a large increase in the activation of calpain B	Drosophila melanogaster

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.22.B37	Ca2+	a 6.4 mM free Ca2+ concentration is required for the half maximal activation of calpain B	Drosophila melanogaster

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.B37	Drosophila melanogaster	Q9VT65	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.22.B37	phosphoprotein	phosphorylation elevates the Ca2+ sensitivity of the protease. The activation of the extracellular signal-regulated protein kinase pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies	Drosophila melanogaster

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.B37	Ni-NTA column chromatography	Drosophila melanogaster

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.22.B37	SCHNEIDER-2 cell	-	Drosophila melanogaster	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.22.B37	microtubule-associated protein 2c + H2O	-	Drosophila melanogaster	?	-	?
3.4.22.B37	N-succinyl-L-Leu-L-Tyr-7-amido-4-methylcoumarin + H2O	-	Drosophila melanogaster	N-succinyl-L-Leu-L-Tyr + 7-amino-4-methyl-coumarin	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.22.B37	calpain B	-	Drosophila melanogaster

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Release 2023.1 (January 2023)