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Literature summary extracted from

  • Derekova, A.; Sjøholm, C.; Mandeva, R.; Michailova, L.; Kambourova, M.
    Biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of Geobacillus stearothermophilus 98 (2006), Extremophiles, 10, 321-326.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.25 CaCl2 1 mM, 1.4fold activation Geobacillus stearothermophilus
4.2.2.25 FeCl2 1 mM, 1.2fold activation Geobacillus stearothermophilus
4.2.2.25 KCl 1 mM, 1.4fold activation Geobacillus stearothermophilus
4.2.2.25 Li2SO4 1 mM, 1.3fold activation Geobacillus stearothermophilus
4.2.2.25 MgCl2 1 mM, 1.4fold activation Geobacillus stearothermophilus
4.2.2.25 MnCl2 1 mM, 1.4fold activation Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.25 Cu2+ 1 mM, 50% inhibition Geobacillus stearothermophilus
4.2.2.25 EDTA 1 mM, 22% inhibition Geobacillus stearothermophilus
4.2.2.25 N-bromosuccinimide 1 mM, 81% inhibition Geobacillus stearothermophilus
4.2.2.25 p-chloromercuribenzoate 1 mM, 39% inhibition Geobacillus stearothermophilus
4.2.2.25 sodium lauryl sulfate 1 mM, 81% inhibition Geobacillus stearothermophilus
4.2.2.25 Urea 1 mM, 22% inhibition Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.25 extracellular the strain produces thermostable gellan lyase extracellularly during exponential phase Geobacillus stearothermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.25 120000
-
 3 * or 4 * 120000, SDS-PAGE Geobacillus stearothermophilus
4.2.2.25 350000
-
 two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis Geobacillus stearothermophilus
4.2.2.25 480000
-
 two protein bands are observed: 350000 Da and 480000 Da, non-denaturing gel electrophoresis Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.25 Geobacillus stearothermophilus P85513 isolated from Bulgarian hot spring
-
4.2.2.25 Geobacillus stearothermophilus 98 P85513 isolated from Bulgarian hot spring
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.25
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.25 0.99
-
 pH 7.0, 70°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.25 gellan
-
 Geobacillus stearothermophilus ?
-
 ?
4.2.2.25 gellan
-
 Geobacillus stearothermophilus 98 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.25 ? 3 * or 4 * 120000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.25 70
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.25 60
-
 24 h, stable Geobacillus stearothermophilus
4.2.2.25 70
-
 half-life: 50 min in absence of substrate. Stable for 2.5 h in presence of substrate Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.25 5 8
-
 Geobacillus stearothermophilus

Expression

EC Number Organism Comment Expression
4.2.2.25 Geobacillus stearothermophilus enzyme activity increases tenfold in conditions of continuous cultivation compared to data from batch fermentations and enzyme productivity is almost sixfold higher up