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Literature summary extracted from

  • Kloeppel, C.; Mueller, A.; Marker, S.; Simon, M.
    Two isoforms of eukaryotic phospholipase C in Paramecium affecting transport and release of GPI-anchored proteins in vivo (2009), Eur. J. Cell Biol., 88, 577-592.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.13 ethanol all PLC1-6 are activated in salt/ethanol extractions Paramecium tetraurelia
4.6.1.13 Salt all PLC1-6 are activated in salt/ethanol extractions Paramecium tetraurelia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.13 cloned into the L4440 vector, transformation of plasmids into Escherichia coli HT115DE3 Paramecium tetraurelia

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.13 p-chloromercuriphenylsulfonic-acid
-
 Paramecium tetraurelia

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 Paramecium tetraurelia
-
 expressing serotype 51A and 51D
-
4.6.1.13 Paramecium tetraurelia d4-2
-
 expressing serotype 51A and 51D
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 additional information all PLCs are able to cleave GPI anchors Paramecium tetraurelia ?
-
 ?
4.6.1.13 additional information all PLCs are able to cleave GPI anchors Paramecium tetraurelia d4-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.13 PI-phospholipase C
-
 Paramecium tetraurelia
4.6.1.13 PI-PLC
-
 Paramecium tetraurelia
4.6.1.13 PLC1
-
 Paramecium tetraurelia
4.6.1.13 PLC2
-
 Paramecium tetraurelia
4.6.1.13 PLC3
-
 Paramecium tetraurelia
4.6.1.13 PLC4
-
 Paramecium tetraurelia
4.6.1.13 PLC5
-
 Paramecium tetraurelia
4.6.1.13 PLC6
-
 Paramecium tetraurelia

Expression

EC Number Organism Comment Expression
4.6.1.13 Paramecium tetraurelia PLC5 reveals a drastic lower mRNA level compared to all other PLCs, this gene shows characteristics of a non-functional pseudogene. Adequate knockdown levels for almost all PLCs in the RNAi lines, except for PLC5 with only 53.85% down
4.6.1.13 Paramecium tetraurelia PLC2, PLC4 and PLC6 show the highest mRNA levels up

General Information

EC Number General Information Comment Organism
4.6.1.13 malfunction with PLC2 and PLC6, RNAi phenotypes show strong defects in release of GPI-anchored surface proteins in vivo Paramecium tetraurelia