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Literature summary extracted from
- Expression and characterization of two functional methionine aminopeptidases from Mycobacterium tuberculosis H37Rv (2009), Curr. Microbiol., 59, 520-525.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.18 | genes Rv0734 and Rv2861c, expression of the His-tagged isozymes in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.18 | Ca2+ | slight inhibition | Mycobacterium tuberculosis |  |
| 3.4.11.18 | Cu2+ | - |
Mycobacterium tuberculosis | |
| 3.4.11.18 | Fe2+ | - |
Mycobacterium tuberculosis | |
| 3.4.11.18 | Ni2+ | - |
Mycobacterium tuberculosis | |
| 3.4.11.18 | Zn2+ | slight inhibition | Mycobacterium tuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.18 | 0.018 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
| 3.4.11.18 | 0.058 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis | |
| 3.4.11.18 | 0.328 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
| 3.4.11.18 | 0.394 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.18 | Co2+ | activates best | Mycobacterium tuberculosis | |
| 3.4.11.18 | Mg2+ | activates slightly | Mycobacterium tuberculosis | |
| 3.4.11.18 | Mn2+ | activates slightly | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.18 | 28812 | - |
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
| 3.4.11.18 | 32516 | - |
x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.18 | Mycobacterium tuberculosis | - |
strains H37Ra and H37Rv, two isozymes MtMetAP1a and MtMetAP1c encoded by genes Rv0734 and Rv2861c | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.18 | recombinant His-tagged isozymes from Escherichia coli, the tag is cleaved off by thrombin | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.18 | Met-Ala-Pro-Thr + H2O | - |
Mycobacterium tuberculosis | Met + Ala-Pro-Thr | - |
? | |
| 3.4.11.18 | Met-Ala-Ser + H2O | - |
Mycobacterium tuberculosis | Met + Ala-Ser | - |
? | |
| 3.4.11.18 | Met-Gly-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Gly-Gly | - |
? | |
| 3.4.11.18 | Met-Gly-Met-Met + H2O | - |
Mycobacterium tuberculosis | Met + Gly-Met-Met | - |
? | |
| 3.4.11.18 | Met-Leu-Phe + H2O | - |
Mycobacterium tuberculosis | Met + Leu-Phe | - |
? | |
| 3.4.11.18 | Met-Phe-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Phe-Gly | - |
? | |
| 3.4.11.18 | Met-Pro-Gly-Gly + H2O | - |
Mycobacterium tuberculosis | Met + Pro-Gly-Gly | - |
? | |
| 3.4.11.18 | additional information | isozyme substrate specificity, overview | Mycobacterium tuberculosis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.18 | ? | x * 28812, isozyme MetAP1a, mass spectrometry, x * 32516, isozyme MetAP1c, mass spectrometry | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.18 | MetAP | - |
Mycobacterium tuberculosis |
| 3.4.11.18 | MetAP1a | - |
Mycobacterium tuberculosis |
| 3.4.11.18 | MetAP1c | - |
Mycobacterium tuberculosis |
| 3.4.11.18 | methionine aminopeptidase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.18 | 37 | - |
isozyme MetAP1c | Mycobacterium tuberculosis |
| 3.4.11.18 | 55 | - |
isozyme MetAP1a | Mycobacterium tuberculosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.18 | 55 | - |
10 min, inactivation of isozyme MetAP1c, while isozyme MetAP1a retains 50% activity | Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.18 | 11.5 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1a | Mycobacterium tuberculosis | |
| 3.4.11.18 | 19.8 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1a, with Co2+ | Mycobacterium tuberculosis | |
| 3.4.11.18 | 39.4 | - |
Met-Gly-Met-Met | pH 7.5, 37°C, isozyme MetAP1c | Mycobacterium tuberculosis | |
| 3.4.11.18 | 74.3 | - |
Met-Ala-Ser | pH 7.5, 37°C, isozyme MetAP1c, with Co2+ | Mycobacterium tuberculosis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.18 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
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