EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.6 | HdLam33, DNA and amino acid sequence determination and analysis | Haliotis discus hannai |
3.2.1.39 | HdLam33, DNA and amino acid sequence determination and analysis | Haliotis discus hannai |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.6 | extracellular | digestive fluid | Haliotis discus hannai | - |
- |
3.2.1.39 | extracellular | - |
Haliotis discus hannai | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 33000 | - |
x * 33000, SDS-PAGE | Haliotis discus hannai |
3.2.1.39 | 33000 | - |
x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation | Haliotis discus hannai |
3.2.1.39 | 36866 | - |
x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation | Haliotis discus hannai |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.6 | laminarin + H2O | Haliotis discus hannai | - |
laminaribiose + D-glucose | - |
? | |
3.2.1.6 | laminaritetraose + 2 H2O | Haliotis discus hannai | - |
laminaribiose + 2 D-glucose | - |
? | |
3.2.1.6 | additional information | Haliotis discus hannai | HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose | ? | - |
? | |
3.2.1.39 | laminarin + H2O | Haliotis discus hannai | - |
laminaribiose + beta-D-glucose | - |
? | |
3.2.1.39 | laminarioligosaccharide + H2O | Haliotis discus hannai | substrate specificities and products, overview | laminaribiose + beta-D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.6 | Haliotis discus hannai | - |
- |
- |
3.2.1.39 | Haliotis discus hannai | C6L684 | Pacific abalone | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.6 | native enzyme 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration to homogeneity | Haliotis discus hannai |
3.2.1.39 | native HdLam33 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration | Haliotis discus hannai |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.6 | digestive juice | - |
Haliotis discus hannai | - |
3.2.1.39 | digestive juice | - |
Haliotis discus hannai | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 68 | - |
purified native enzyme | Haliotis discus hannai |
3.2.1.39 | 68 | - |
purified enzyme | Haliotis discus hannai |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.6 | laminarin + H2O | - |
Haliotis discus hannai | laminaribiose + D-glucose | - |
? | |
3.2.1.6 | laminaritetraose + 2 H2O | - |
Haliotis discus hannai | laminaribiose + 2 D-glucose | - |
? | |
3.2.1.6 | laminaritetraose + 2 H2O | from Laminaria digitata. HdLam33 performs a transglycosylation reaction transferring the laminaribiose unit in the non-reducing terminus of laminaritriose , as donor substrate, to a free laminaribiose, as acceptor substrate, giving laminaritetraose and glucose. The resulting laminaritetraose is subsequently hydrolyzed by HdLam33 into 2 mol of glucose and 1 mol of laminaribiose | Haliotis discus hannai | laminaribiose + 2 D-glucose | - |
? | |
3.2.1.6 | lichenan + H2O | from Cetraria islandica. HdLam33 produces no cellotriose, cellobiose, and glucose from lichenan, but produced the trisaccharide cellobiosyl-beta-1,3-glucose | Haliotis discus hannai | beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranose + ? | - |
? | |
3.2.1.6 | additional information | HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose | Haliotis discus hannai | ? | - |
? | |
3.2.1.39 | laminarin + H2O | - |
Haliotis discus hannai | laminaribiose + beta-D-glucose | - |
? | |
3.2.1.39 | laminarioligosaccharide + H2O | - |
Haliotis discus hannai | laminaribiose + beta-D-glucose | - |
? | |
3.2.1.39 | laminarioligosaccharide + H2O | substrate specificities and products, overview | Haliotis discus hannai | laminaribiose + beta-D-glucose | - |
? | |
3.2.1.39 | additional information | HdLam33 possesses transglycosylation activity splitting glycoside linkage in a retaining manner, overview. It also shows activity of EC 3.2.1.6, endo-1,3(4)-beta-glucanase | Haliotis discus hannai | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.6 | ? | x * 33000, SDS-PAGE | Haliotis discus hannai |
3.2.1.6 | More | HdLam33 primary structure, overview | Haliotis discus hannai |
3.2.1.39 | ? | x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation | Haliotis discus hannai |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.6 | beta-1,3-glucanase | - |
Haliotis discus hannai |
3.2.1.6 | HdLam33 | - |
Haliotis discus hannai |
3.2.1.6 | More | HdLam33 belongs to the glycoside hydrolase family 16, GH16 | Haliotis discus hannai |
3.2.1.39 | beta-1,3-glucanase | - |
Haliotis discus hannai |
3.2.1.39 | endo-1,3;4-beta-glucanase | - |
Haliotis discus hannai |
3.2.1.39 | HdLam33 | - |
Haliotis discus hannai |
3.2.1.39 | More | the enzyme belongs to the glycoside hydrolase family 16 | Haliotis discus hannai |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 50 | - |
- |
Haliotis discus hannai |
3.2.1.39 | 50 | - |
- |
Haliotis discus hannai |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 40 | 70 | - |
Haliotis discus hannai |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 50 | - |
purified native enzyme, 15 min, loss of 50% activity | Haliotis discus hannai |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.6 | 6 | - |
- |
Haliotis discus hannai |
3.2.1.39 | 6 | - |
- |
Haliotis discus hannai |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 4 | 9.5 | - |
Haliotis discus hannai |