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Literature summary extracted from

  • Kumagai, Y.; Ojima, T.
    Enzymatic properties and the primary structure of a beta-1,3-glucanase from the digestive fluid of the Pacific abalone Haliotis discus hannai (2009), Comp. Biochem. Physiol. B, 154, 113-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 HdLam33, DNA and amino acid sequence determination and analysis Haliotis discus hannai
3.2.1.39 HdLam33, DNA and amino acid sequence determination and analysis Haliotis discus hannai

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.6 extracellular digestive fluid Haliotis discus hannai
-
-
3.2.1.39 extracellular
-
Haliotis discus hannai
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 33000
-
x * 33000, SDS-PAGE Haliotis discus hannai
3.2.1.39 33000
-
x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation Haliotis discus hannai
3.2.1.39 36866
-
x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation Haliotis discus hannai

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.6 laminarin + H2O Haliotis discus hannai
-
laminaribiose + D-glucose
-
?
3.2.1.6 laminaritetraose + 2 H2O Haliotis discus hannai
-
laminaribiose + 2 D-glucose
-
?
3.2.1.6 additional information Haliotis discus hannai HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose ?
-
?
3.2.1.39 laminarin + H2O Haliotis discus hannai
-
laminaribiose + beta-D-glucose
-
?
3.2.1.39 laminarioligosaccharide + H2O Haliotis discus hannai substrate specificities and products, overview laminaribiose + beta-D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Haliotis discus hannai
-
-
-
3.2.1.39 Haliotis discus hannai C6L684 Pacific abalone
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 native enzyme 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration to homogeneity Haliotis discus hannai
3.2.1.39 native HdLam33 340fold from digestive fluid by ammonium sulfate fractionation, hydrophobic interaction and cation exchange chromatography, and gel filtration Haliotis discus hannai

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.6 digestive juice
-
Haliotis discus hannai
-
3.2.1.39 digestive juice
-
Haliotis discus hannai
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 68
-
purified native enzyme Haliotis discus hannai
3.2.1.39 68
-
purified enzyme Haliotis discus hannai

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 laminarin + H2O
-
Haliotis discus hannai laminaribiose + D-glucose
-
?
3.2.1.6 laminaritetraose + 2 H2O
-
Haliotis discus hannai laminaribiose + 2 D-glucose
-
?
3.2.1.6 laminaritetraose + 2 H2O from Laminaria digitata. HdLam33 performs a transglycosylation reaction transferring the laminaribiose unit in the non-reducing terminus of laminaritriose , as donor substrate, to a free laminaribiose, as acceptor substrate, giving laminaritetraose and glucose. The resulting laminaritetraose is subsequently hydrolyzed by HdLam33 into 2 mol of glucose and 1 mol of laminaribiose Haliotis discus hannai laminaribiose + 2 D-glucose
-
?
3.2.1.6 lichenan + H2O from Cetraria islandica. HdLam33 produces no cellotriose, cellobiose, and glucose from lichenan, but produced the trisaccharide cellobiosyl-beta-1,3-glucose Haliotis discus hannai beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranose + ?
-
?
3.2.1.6 additional information HdLam33 degrades laminarin and laminarioligosaccharides to laminaribiose and glucose Haliotis discus hannai ?
-
?
3.2.1.39 laminarin + H2O
-
Haliotis discus hannai laminaribiose + beta-D-glucose
-
?
3.2.1.39 laminarioligosaccharide + H2O
-
Haliotis discus hannai laminaribiose + beta-D-glucose
-
?
3.2.1.39 laminarioligosaccharide + H2O substrate specificities and products, overview Haliotis discus hannai laminaribiose + beta-D-glucose
-
?
3.2.1.39 additional information HdLam33 possesses transglycosylation activity splitting glycoside linkage in a retaining manner, overview. It also shows activity of EC 3.2.1.6, endo-1,3(4)-beta-glucanase Haliotis discus hannai ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 33000, SDS-PAGE Haliotis discus hannai
3.2.1.6 More HdLam33 primary structure, overview Haliotis discus hannai
3.2.1.39 ? x * 33000, HdLam33, SDS-PAGE, x * 36866, Hdlam33, sequence calculation Haliotis discus hannai

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-1,3-glucanase
-
Haliotis discus hannai
3.2.1.6 HdLam33
-
Haliotis discus hannai
3.2.1.6 More HdLam33 belongs to the glycoside hydrolase family 16, GH16 Haliotis discus hannai
3.2.1.39 beta-1,3-glucanase
-
Haliotis discus hannai
3.2.1.39 endo-1,3;4-beta-glucanase
-
Haliotis discus hannai
3.2.1.39 HdLam33
-
Haliotis discus hannai
3.2.1.39 More the enzyme belongs to the glycoside hydrolase family 16 Haliotis discus hannai

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 50
-
-
Haliotis discus hannai
3.2.1.39 50
-
-
Haliotis discus hannai

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.39 40 70
-
Haliotis discus hannai

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 50
-
purified native enzyme, 15 min, loss of 50% activity Haliotis discus hannai

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 6
-
-
Haliotis discus hannai
3.2.1.39 6
-
-
Haliotis discus hannai

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4 9.5
-
Haliotis discus hannai