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Literature summary extracted from

  • Omori, T.; Ueno, K.; Muramatsu, K.; Kikuchi, M.; Onodera, S.; Shiomi, N.
    Characterization of recombinant beta-fructofuranosidase from Bifidobacterium adolescentis G1 (2010), Chem. Cent. J., 4, 09.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 deoxycholic acid 10 mM, 137% of initial activity activity Bifidobacterium adolescentis
3.2.1.26 Triton X-100 10 mM, 138% of initial activity activity Bifidobacterium adolescentis
3.2.1.26 Tween-80 10 mM, 138% of initial activity activity Bifidobacterium adolescentis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 expression in Escherichia coli Bifidobacterium adolescentis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Ag+ 10 mM, no residual activity Bifidobacterium adolescentis
3.2.1.26 Cu2+ 10 mM, 52% residual activity Bifidobacterium adolescentis
3.2.1.26 Hg2+ 10 mM, no residual activity Bifidobacterium adolescentis
3.2.1.26 Sodium dodecyl sulfate 10 mM, no residual activity Bifidobacterium adolescentis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 1.7
-
 1-kestose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 1.7
-
 neokestose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 2.2
-
 fructosylnystose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 3.9
-
 nystose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 25.9
-
 inulin pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 38
-
 sucrose pH 5.7, 37°C Bifidobacterium adolescentis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60300
-
 x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS Bifidobacterium adolescentis
3.2.1.26 66000
-
 x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS Bifidobacterium adolescentis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Bifidobacterium adolescentis
-
-
-
3.2.1.26 Bifidobacterium adolescentis G1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 86
-
 pH 5.7, 37°C Bifidobacterium adolescentis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 1-kestose + H2O
-
 Bifidobacterium adolescentis D-fructose + ?
-
 ?
3.2.1.26 1-kestose + H2O
-
 Bifidobacterium adolescentis G1 D-fructose + ?
-
 ?
3.2.1.26 fructosylnystose + H2O
-
 Bifidobacterium adolescentis D-fructose + ?
-
 ?
3.2.1.26 inulin + H2O
-
 Bifidobacterium adolescentis D-fructose + ?
-
 ?
3.2.1.26 inulin + H2O
-
 Bifidobacterium adolescentis G1 D-fructose + ?
-
 ?
3.2.1.26 neokestose + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
3.2.1.26 neokestose + H2O
-
 Bifidobacterium adolescentis G1 ?
-
 ?
3.2.1.26 nystose + H2O
-
 Bifidobacterium adolescentis D-fructose + ?
-
 ?
3.2.1.26 nystose + H2O
-
 Bifidobacterium adolescentis G1 D-fructose + ?
-
 ?
3.2.1.26 sucrose + H2O
-
 Bifidobacterium adolescentis beta-D-fructose + alpha-D-glucose
-
 ?
3.2.1.26 sucrose + H2O
-
 Bifidobacterium adolescentis G1 beta-D-fructose + alpha-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS Bifidobacterium adolescentis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 50
-
 stable up to Bifidobacterium adolescentis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 75.4
-
 fructosylnystose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 77.4
-
 inulin pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 79.4
-
 sucrose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 107
-
 1-kestose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 142.7
-
 neokestose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 152.8
-
 nystose pH 5.7, 37°C Bifidobacterium adolescentis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 5.7
-
-
 Bifidobacterium adolescentis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 5 8.6
-
 Bifidobacterium adolescentis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.26 2.1
-
 sucrose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 3
-
 inulin pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 34.2
-
 fructosylnystose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 39.2
-
 nystose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 63.2
-
 1-kestose pH 5.7, 37°C Bifidobacterium adolescentis
3.2.1.26 83.9
-
 neokestose pH 5.7, 37°C Bifidobacterium adolescentis