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Literature summary extracted from
- Biosynthetic pathways of the endocannabinoid anandamide (2007), Chem. Biodivers., 4, 1842-1857.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.54 | - |
Mus musculus |
| 3.1.4.54 | - |
Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.54 | C224S | considerable reduction in activity | Rattus norvegicus |
| 3.1.4.54 | H380R | considerable reduction in activity | Rattus norvegicus |
| 3.1.4.54 | L207F | considerable reduction in activity | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.54 | p-chloromercuribenzoate | - |
Rattus norvegicus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.54 | Zn2+ | presence of of catalytically crucial Zn2+, NAPE-PLD is a member of the metallo-beta-lactamase family | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.54 | 46000 | - |
x * 46000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.54 | N-arachidonoyl-phosphatidylethanolamine + H2O | Mus musculus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
| 3.1.4.54 | N-arachidonoylphosphatidylethanolamine + H2O | Rattus norvegicus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.54 | Homo sapiens | Q6IQ20 | - |
- |
| 3.1.4.54 | Mus musculus | Q8BH82 | - |
- |
| 3.1.4.54 | Rattus norvegicus | Q769K2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.54 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.54 | brain | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
| 3.1.4.54 | brain | widely distributed in various brain regions of rat, with the highest activity in the brain stem. The enzyme activity in rat brain changes during development, and infant rats show a several-fold higher activity than adult rats | Rattus norvegicus | - |
| 3.1.4.54 | kidney | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
| 3.1.4.54 | oviduct | NAPE-PLD is expressed in mouse oviduct on days one to four of pregnancy | Mus musculus | - |
| 3.1.4.54 | testis | - |
Rattus norvegicus | - |
| 3.1.4.54 | testis | activity detected in almost all of the mouse organs, with higher specific activities in brain, kidney, and testis | Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.54 | N-arachidonoyl-phosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
| 3.1.4.54 | N-arachidonoylphosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | Rattus norvegicus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.54 | ? | x * 46000, SDS-PAGE | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.54 | NAPE-PLD | - |
Mus musculus |
| 3.1.4.54 | NAPE-PLD | - |
Homo sapiens |
| 3.1.4.54 | NAPE-PLD | - |
Rattus norvegicus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.1.4.54 | Rattus norvegicus | the enzyme appears to be constitutively active | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.4.54 | malfunction | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus |
| 3.1.4.54 | physiological function | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines. Analysis of NAPE-PLD-deficient mice reveals the presence of NAPE-PLD independent pathways for the anandamide formation | Mus musculus |
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