BRENDA - Enzyme Database

Role of sphingosine kinases and lipid phosphate phosphatases in regulating spatial sphingosine 1-phosphate signalling in health and disease

Pyne, S.; Lee, S.C.; Long, J.; Pyne, N.J.; Cell. Signal. 21, 14-21 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
is N-ethylmaleimide insensitive
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
LPP1, LPP2 and LPP3
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
R127K/H223L
negligible LPP1 activity, although each mutant is still able to form oligomers
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
LPPs are integral membrane proteins with six transmembrane domains
Homo sapiens
16020
-
3.1.3.4
additional information
LPP1 and LPP3 are present in caveolae, localised to distinct lipid rafts
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
is Mg2+ independent
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Drosophila melanogaster
-
-
-
3.1.3.4
Homo sapiens
-
-
-
3.1.3.4
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
central nervous system
-
Drosophila melanogaster
-
3.1.3.4
embryonic fibroblast
-
Mus musculus
-
3.1.3.4
posterior midgut
-
Drosophila melanogaster
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
-
708068
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
708068
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
the catalytic site is arranged into three distinct domains: one substrate recognition site and two catalytic sites
708068
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
708068
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
708068
Homo sapiens
sphingosine + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
dimer
catalytic activity is required for dimerisation
Drosophila melanogaster
3.1.3.4
hexamer
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
is N-ethylmaleimide insensitive
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
LPP1, LPP2 and LPP3
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
R127K/H223L
negligible LPP1 activity, although each mutant is still able to form oligomers
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
LPPs are integral membrane proteins with six transmembrane domains
Homo sapiens
16020
-
3.1.3.4
additional information
LPP1 and LPP3 are present in caveolae, localised to distinct lipid rafts
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
is Mg2+ independent
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
central nervous system
-
Drosophila melanogaster
-
3.1.3.4
embryonic fibroblast
-
Mus musculus
-
3.1.3.4
posterior midgut
-
Drosophila melanogaster
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
ceramide 1-phosphate + H2O
-
708068
Homo sapiens
ceramide + phosphate
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
708068
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
the catalytic site is arranged into three distinct domains: one substrate recognition site and two catalytic sites
708068
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
708068
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
708068
Homo sapiens
sphingosine + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
dimer
catalytic activity is required for dimerisation
Drosophila melanogaster
3.1.3.4
hexamer
-
Homo sapiens
Expression
EC Number
Organism
Commentary
Expression
3.1.3.4
Homo sapiens
epidermal growth factor increases LPP3 mRNA in HeLa cells. Androgen increases LPP1 mRNA in adenocarcinoma cells
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.4
physiological function
wunen generates a phospholipid gradient and thereby repels germ cells towards higher phospholipid levels and away from the midline. Wunen/wunen-2 also governs the death of mis-migrating germ cells and survival of pole cells which compete for a common phospholipid substrate with somatic cells
Drosophila melanogaster
3.1.3.4
physiological function
stable overexpression of LPP1 or LPP2 reduces sphingosine 1-phosphate, lysophosphatidic acid- and thrombin-induced activation of ERK-1/2
Homo sapiens
3.1.3.4
physiological function
physiologically relevant increases in LPP1 activity in mouse embryonic fibroblasts (isolated from transgenic mice with 20 gene copies of LPP1) reduces lysophosphatidic acid- and platelet-derived growth factor-activation of ERK-1/2 and migration, resulting from down-regulation of typical proteinkinase C isoform(s) which are required for regulation of cell migration
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.4
physiological function
wunen generates a phospholipid gradient and thereby repels germ cells towards higher phospholipid levels and away from the midline. Wunen/wunen-2 also governs the death of mis-migrating germ cells and survival of pole cells which compete for a common phospholipid substrate with somatic cells
Drosophila melanogaster
3.1.3.4
physiological function
stable overexpression of LPP1 or LPP2 reduces sphingosine 1-phosphate, lysophosphatidic acid- and thrombin-induced activation of ERK-1/2
Homo sapiens
3.1.3.4
physiological function
physiologically relevant increases in LPP1 activity in mouse embryonic fibroblasts (isolated from transgenic mice with 20 gene copies of LPP1) reduces lysophosphatidic acid- and platelet-derived growth factor-activation of ERK-1/2 and migration, resulting from down-regulation of typical proteinkinase C isoform(s) which are required for regulation of cell migration
Mus musculus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.4
Homo sapiens
epidermal growth factor increases LPP3 mRNA in HeLa cells. Androgen increases LPP1 mRNA in adenocarcinoma cells
up