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Literature summary extracted from

  • Struszczyk, K.; Szczesna-Antczak, M.; Walczak, M.; Pomianowska, E.; Antczak, T.
    Isolation and purification of Mucor circinelloides intracellular chitosanolytic enzymes (2009), Carbohydr. Polym., 78, 16-24.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Ag+ 89% inhibition at 1 mM, 79% at 0.5 mM Mucor circinelloides
3.2.1.132 Ba2+ 11% inhibition at 1 mM Mucor circinelloides
3.2.1.132 Cd2+ 13% inhibition at 1 mM Mucor circinelloides
3.2.1.132 Co2+ 13% inhibition at 1 mM Mucor circinelloides
3.2.1.132 Cu2+ 92% inhibition at 1 mM, 82% at 0.5 mM Mucor circinelloides
3.2.1.132 EDTA 17% inhibition at 1% w/v, 82% at 5% w/v Mucor circinelloides
3.2.1.132 Fe2+ 26% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides
3.2.1.132 Hg2+ complete inhibition at 1 mM Mucor circinelloides
3.2.1.132 Pb2+ 14% inhibition at 1 mM, 8% at 0.5 mM Mucor circinelloides
3.2.1.132 SDS 82% inhibition at 1% w/v Mucor circinelloides
3.2.1.132 Sn2+ 20% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides
3.2.1.132 Triton X-100 80% inhibition at 1% w/v Mucor circinelloides
3.2.1.132 Tween 20 72% inhibition at 1% w/v Mucor circinelloides
3.2.1.132 Tween 80 79% inhibition at 1% w/v Mucor circinelloides
3.2.1.132 Zn2+ 24% inhibition at 1 mM, 9% at 0.5 mM Mucor circinelloides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 intracellular
-
 Mucor circinelloides 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ activates Mucor circinelloides
3.2.1.132 Mg2+ activates Mucor circinelloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 42000
-
 x * 42000, SDS-PAGE Mucor circinelloides

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Mucor circinelloides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 native enzyme 23fold from mycelium by bacitracin affinity chromatography and gel filtration Mucor circinelloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 mycelium air-dried and defatted Mucor circinelloides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O the chitosanase is an endo-type enzyme, the preferred substrates of chitosanase are chitosan preparations with a high degree of deacetylation, substrate is chitosan 66-97% deacetylated Mucor circinelloides ?
-
 ?
3.2.1.132 additional information The enzyme might be identical with a lipase that co-purifies with the chitosanase. No activity for colloidal chitin, Na-CMC and starch Mucor circinelloides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 42000, SDS-PAGE Mucor circinelloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 37
-
-
 Mucor circinelloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 20 50 15-20% of maximal activity at 20°C, 30% at 50°C, temperature profile, overview Mucor circinelloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5 6
-
 Mucor circinelloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 7 20% of maximal activity at pH 4.0, 10% at pH 7.0, pH profile, overview Mucor circinelloides