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Literature summary extracted from

  • Kheder, F.; Delaunay, S.; Abo-Chameh, G.; Paris, C.; Muniglia, L.; Girardin, M.
    Production and biochemical characterization of a type B ferulic acid esterase from Streptomyces ambofaciens (2009), Can. J. Microbiol., 55, 729-738.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 43000
-
x * 43000, SDS-PAGE, partially purified enzyme Streptomyces ambofaciens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 no activity in Streptomyces lividans
-
-
-
3.1.1.73 no activity in Streptomyces lividans TK24
-
-
-
3.1.1.73 no activity in Streptomyces pristinaespiralis
-
-
-
3.1.1.73 no activity in Streptomyces pristinaespiralis Pr11
-
-
-
3.1.1.73 Streptomyces ambofaciens
-
strain ATCC 23877
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 partially purified by 50% ammonium sulfate precipitation and dialysis, 3.6fold with a yield of 25% Streptomyces ambofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 40
-
with destarched maize bran (1%) as the carbon source, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 40
-
with rape cattle cake (1%) as the carbon source, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 60
-
with destarched wheat bran (1%) as the carbon source and in the presence of 0.17 g/L ferulic acid, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 100
-
FAE activity decreases up to 24 h of culture and is maintained at 0.1 mU/mg protein with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 160
-
with methyl cinnamate as substrate, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 200
-
with destarched wheat bran (1%) as the carbon source and in the presence of 0.03 g/L ferulic acid, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 200
-
with methyl ferulate as substrate, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 210
-
with oat spelt xylan (1%) as the carbon source, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 220
-
with destarched wheat bran (1%) as the carbon source or ethyl cinnamate as substrate, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 300
-
with methyl p-coumarate as substrate, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 600
-
after 14 h of culture with destarched wheat bran (1%) as the carbon source, at pH 6.5 and 40°C Streptomyces ambofaciens
3.1.1.73 800
-
3.6fold purified enzyme Streptomyces ambofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl cinnamate + H2O highest activity Streptomyces ambofaciens cinnamic acid + methanol
-
?
3.1.1.73 methyl ferulate + H2O highest activity Streptomyces ambofaciens ferulic acid + methanol
-
?
3.1.1.73 methyl p-coumarate + H2O highest activity Streptomyces ambofaciens p-coumaric acid + methanol
-
?
3.1.1.73 additional information no activity with butyl cinnamate, methyl caffeate, methyl sinapate, methyl vanillate and methyl syringate Streptomyces ambofaciens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 43000, SDS-PAGE, partially purified enzyme Streptomyces ambofaciens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
Streptomyces ambofaciens
3.1.1.73 ferulic acid esterase
-
Streptomyces ambofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
purified enzyme Streptomyces ambofaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 60 purified enzyme Streptomyces ambofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 37
-
after 2 h at 37°C, the FAE specific activity is 60% reduced Streptomyces ambofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
purified enzyme Streptomyces ambofaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 9 purified enzyme Streptomyces ambofaciens

Expression

EC Number Organism Comment Expression
3.1.1.73 Streptomyces ambofaciens no FAE activity in the presence of 0.1% ethyl ferulate in the culture medium. Low FAE activity in the absence of lignocellulosic substrate, maybe due to a constitutive synthesis of the FAE down
3.1.1.73 Streptomyces ambofaciens induction of the FAE synthesis is not related to the ferulic acid content in the substrate used additional information
3.1.1.73 Streptomyces ambofaciens maximal level of activity in the presence of destarched wheat bran as the sole carbon source. 1% (m/v) of destarched wheat bran is the optimal concentration to induce its production. With this inducer, no ferulic acid dimers are released from the cell wall by the produced FAE. Destarched maize bran is capable of inducing the synthesis of a small amount of FAE. Maximal level of activity in the presence of oat spelt xylan as the sole carbon source. Rape cattle cake (Brassica napus), which does not contain esterified ferulic acid, is capable of inducing the synthesis of a small amount of FAE. With beet pectins or with white dextrins, FAE activity is detected when a drop of culture supernatant is deposited on the agar medium containing ethyl ferulate, but this activity is too weak to be quantified up