Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garcia, L.M.; Contreras-Zentella, M.L.; Jaramillo, R.; Benito-Mercade, M.C.; Mendoza-Hernandez, G.; del Arenal, I.P.; Membrillo-Hernandez, J.; Escamilla, J.E.
    The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme (2008), Can. J. Microbiol., 54, 456-466.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-n-heptyl-4-hydroxyquinolino-N-oxide
-
 Bacillus cereus
1.3.5.1 malonate competitive Bacillus cereus
1.3.5.1 additional information not inhibitory: thenoyltrifluoroacetone, 2-n-heptyl-4-hydroxyquinoline N-oxide Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.011
-
 decylubiquinone pH 7.6, 30°C Bacillus cereus
1.3.5.1 0.33
-
 phenazine methosulfate pH 7.6, 30°C Bacillus cereus
1.3.5.1 0.64
-
 succinate pH 7.6, 30°C Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 membrane
-
 Bacillus cereus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Bacillus cereus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.5.1 succinate + a quinone = fumarate + a quinol reduction of menaquinone-7 is the rate-limiting step in succinate respiration Bacillus cereus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.1 0.0076
-
 electron accepotr 2,6-dichlorophenolindophenol, pH 7.6, 30°C Bacillus cereus
1.3.5.1 0.0076
-
 electron acceptor 2,6-dichlorophenolindophenol, pH 7.6, 30°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + reduced decylubiquinone
-
 Bacillus cereus succinate + decylubiquinone
-
 r
1.3.5.1 additional information enzyme also accepts reduced decylubiquinone, reaction of EC 1.3.5.1 Bacillus cereus ?
-
 ?
1.3.5.1 additional information enzyme also accetps phenazine methosulfate, reaction of EC 1.3.5.4 Bacillus cereus ?
-
 ?
1.3.5.1 succinate + decylubiquinone
-
 Bacillus cereus fumarate + reduced decylubiquinone
-
 r
1.3.5.1 succinate + phenazine methosulfate
-
 Bacillus cereus fumarate + reduced phenazine methosulfate
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.5.1 ? enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 SQR
-
 Bacillus cereus
1.3.5.1 SQR ambiguous Bacillus cereus
1.3.5.1 succinate:menaquinone 7-reductase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.1 30
-
 oxidation of succinate, assay at Bacillus cereus
1.3.5.1 30
-
 reduction of fumarate, assay at Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 77
-
 phenazine methosulfate pH 7.6, 30°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 7.8
-
 with decylubiquinone-dichlorophenolindophenol as electron acceptor Bacillus cereus
1.3.5.1 7.8
-
 with phenazine methosulfate-dichlorophenolindophenyl as electron acceptor Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 FAD
-
 Bacillus cereus
1.3.5.1 heme
-
 Bacillus cereus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.5.1 0.00062
-
 2-n-heptyl-4-hydroxyquinolino-N-oxide pH 7.6, 30°C Bacillus cereus
1.3.5.1 0.088
-
 malonate pH 7.6, 30°C Bacillus cereus