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Literature summary extracted from

  • James, L.R.; Griffiths, C.H.; Garthwaite, J.; Bellamy, T.C.
    Inhibition of nitric oxide-activated guanylyl cyclase by calmodulin antagonists (2009), Br. J. Pharmacol., 158, 1454-1464.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.2 nitric oxide
-
 Bos taurus
4.6.1.2 protoporphyrin IX 0.001 mM activates untreated sGC with around half the effectiveness of nitric oxide Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.2 Ca2+ ionophore A23187 Ca2+-dependent inhibition of sGC can be achieved with the Ca2+ ionophore A23187 (0.01 mM) Bos taurus
4.6.1.2 Calmidazolium uncompetitive inhibition, time-dependent inhibition at 0.03 mM, pre-treatment of sGC with calmidazolium, followed by filtration, causes an 80% inhibition of sGC activation by nitric oxide and a 23% inhibition of activation by protoporphyrin IX Bos taurus
4.6.1.2 N-benzyl-N-(2-chloroethyl)-1-phenoxypropan-2-amine
-
 Bos taurus
4.6.1.2 Trifluoperazine
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.2 GTP Bos taurus
-
 3',5'-cyclic-GMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.2 lung
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Bos taurus 3',5'-cyclic-GMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 alpha1beta1 guanylyl cyclase
-
 Bos taurus
4.6.1.2 sGC
-
 Bos taurus
4.6.1.2 soluble guanylyl cyclase
-
 Bos taurus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.6.1.2 0.01
-
 purified sGC Bos taurus Calmidazolium

Expression

EC Number Organism Comment Expression
4.6.1.2 Bos taurus pre-treatment of sGC with Tween-20 (0.5%), followed by filtration to remove the detergent, decreases the effectiveness of nitric oxide for activating cGMP synthesis by 73% down