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Literature summary extracted from

  • He, J.; Yin, J.; Wang, L.; Yu, B.; Chen, D.
    Functional characterisation of a recombinant xylanase from Pichia pastoris and effect of the enzyme on nutrient digestibility in weaned pigs (2010), Br. J. Nutr., 103, 1507-1513.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 agriculture unprocessed Xyn2, supplemented to a xylan-containing diet for weaned pigs, results an average body-weight gain increased by 16.9% with added Xyn2 at a concentration of 500 U/kg diet Trichoderma reesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xyn2, expression in Pichia pastoris strain PX-1 Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 21000
-
 x * 21000, Xyn2, SDS-PAGE Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Trichoderma reesei
-
 gene xyn2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant Xyn2 from Pichia pastoris strain PX-1 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O from oat spelt, birchwood, or beechwood, specificity, overview Trichoderma reesei xylotriose + xylobiose + ? as the main degradation products of Xyn2 ?
3.2.1.8 additional information Xyn2 shows no activity with gellan gum, Avicel, and carboxymethyl cellulose Trichoderma reesei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 21000, Xyn2, SDS-PAGE Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Trichoderma reesei
3.2.1.8 xylanase 2
-
 Trichoderma reesei
3.2.1.8 Xyn2
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
 Xyn2 Trichoderma reesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 20 90 activity range, profile, overview Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 purified enzyme, 30 min, loss of less that 10% activity Trichoderma reesei
3.2.1.8 60
-
 purified enzyme, 30 min, loss of about 70% activity Trichoderma reesei
3.2.1.8 70
-
 purified enzyme, 30 min, loss of over 90% activity Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 339.6
-
 beta-1,4-xylan beechwood xylan, pH 6.0, 60°C, recombinant Xyn2 Trichoderma reesei
3.2.1.8 435.7
-
 beta-1,4-xylan birchwood xylan, pH 6.0, 60°C, recombinant Xyn2 Trichoderma reesei
3.2.1.8 512.4
-
 beta-1,4-xylan oat spelt xylan, pH 6.0, 60°C, recombinant Xyn2 Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
 Xyn2 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3 8 activity range, profile, overview Trichoderma reesei