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Literature summary extracted from

  • Kim, J.H.; Kim, H.R.; Lim, M.H.; Ko, H.M.; Chin, J.E.; Lee, H.B.; Kim, I.C.; Bai, S.
    Construction of a direct starch-fermenting industrial strain of Saccharomyces cerevisiae producing glucoamylase, alpha-amylase and debranching enzyme (2010), Biotechnol. Lett., 32, 713-719.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli Schwanniomyces occidentalis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 additional information gene for alpha-amylase from Debaryomyces occidentalis was integrated into genom of Saccharomyces cerevisiae Schwanniomyces occidentalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 55000
-
 SDS-PAGE Schwanniomyces occidentalis
3.2.1.3 83000
-
 SDS-PAGE Aspergillus awamori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Schwanniomyces occidentalis
-
 gene for alpha-amylase from Debaryomyces occidentalis was integrated into genome of Saccharomyces cerevisiae
-
3.2.1.3 Aspergillus awamori
-
 gene of glucoamylase from Aspergillus awamori integrated into genome of Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
 values about 5.8 U/ml for different transformants Schwanniomyces occidentalis
3.2.1.3 additional information
-
 0.12 U/ml, substrate pullulan Aspergillus awamori
3.2.1.3 additional information
-
 0.51 U/ml, substrate isomaltose Aspergillus awamori
3.2.1.3 additional information
-
 values between 0.56 to 1.02 U/ml for different transformants Aspergillus awamori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 isomaltose + H2O assay at pH 5.5, 40°C Aspergillus awamori ?
-
 ?
3.2.1.3 pullulan + H2O assay at pH 5.5, 40°C Aspergillus awamori ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 alpha-amylase
-
 Schwanniomyces occidentalis
3.2.1.1 Amy
-
 Schwanniomyces occidentalis
3.2.1.3 GA1
-
 Aspergillus awamori
3.2.1.3 glucoamylase
-
 Aspergillus awamori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 40
-
 assay at Schwanniomyces occidentalis
3.2.1.3 40
-
 assay at Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5
-
 assay at Schwanniomyces occidentalis
3.2.1.3 5.5
-
 assay at Aspergillus awamori