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Literature summary extracted from

  • Lee, J.; Heo, S.; Lee, J.; Yoon, K.; Kim, Y.; Nam, S.
    Thermostability and xylan-hydrolyzing property of endoxylanase expressed in yeast Saccharomyces cerevisiae (2009), Biotechnol. Bioprocess Eng., 14, 639-644.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynB, expression in Saccharomyces cerevisiae strain SEY2102, the recombinant enzyme is secreted, and the extracellular endoxylanase expressed in yeast shows an enhanced thermal stability due to the N-linked glycosylation Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
Bacillus subtilis
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus subtilis Q45VU2 xynB; gene xynB
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein the extracellular endoxylanase expressed in yeast shows an enhanced thermal stability due to the N-linked glycosylation, the native enzyme in Bacillus subtilis is not glycosylated Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O from birchwood Bacillus subtilis xylobiose + xylotriose + ? major products with equimolar ratio ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 beta-1,4-endoxylanase
-
Bacillus subtilis
3.2.1.8 endoxylanase
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
assay at Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
glycosylated recombinant enzyme, 30 h, 50% remaining activity. Unglycosylated native enzyme, 12 h, 50% activity remaining Bacillus subtilis
3.2.1.8 70
-
glycosylated recombinant enzyme, 1 h, 90% remaining activity Bacillus subtilis
3.2.1.8 80
-
glycosylated recombinant enzyme, 1 h, 86% remaining activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
assay at Bacillus subtilis