EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.49 | Ag+ | moderate inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II | Asterina amurensis | |
3.2.1.49 | Ag+ | - |
Homo sapiens | |
3.2.1.49 | Ca2+ | - |
Homo sapiens | |
3.2.1.49 | Cu2+ | - |
Homo sapiens | |
3.2.1.49 | D-galactose | about 10% inhibition of isozymes alpha-GalNAcase I and isozyme alpha-GalNAcase II | Asterina amurensis | |
3.2.1.49 | D-galactose | - |
Homo sapiens | |
3.2.1.49 | D-glucose | - |
Homo sapiens | |
3.2.1.49 | Hg2+ | slight inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II | Asterina amurensis | |
3.2.1.49 | Hg2+ | - |
Homo sapiens | |
3.2.1.49 | K+ | - |
Homo sapiens | |
3.2.1.49 | Mg2+ | - |
Homo sapiens | |
3.2.1.49 | Mn2+ | - |
Homo sapiens | |
3.2.1.49 | additional information | no inhibition by D-glucose, N-acetyl-D-glucosamine, and Mn2+ | Asterina amurensis | |
3.2.1.49 | N-acetyl-D-galactosamine | 57% and 62% inhibition of isozyme alpha-GalNAcase I and isozyme alpha-GalNAcase II | Asterina amurensis | |
3.2.1.49 | N-acetyl-D-galactosamine | - |
Homo sapiens | |
3.2.1.49 | N-acetyl-D-glucosamine | - |
Homo sapiens | |
3.2.1.49 | Na+ | - |
Homo sapiens | |
3.2.1.49 | Zn2+ | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.49 | 1.04 | - |
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide | pH 4.2, 37°C, isozyme alpha-GalNAcase I | Asterina amurensis | |
3.2.1.49 | 1.79 | - |
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide | pH 4.2, 37°C | Homo sapiens | |
3.2.1.49 | 3.97 | - |
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide | pH 4.2, 37°C, isozyme alpha-GalNAcase II | Asterina amurensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.49 | Ca2+ | slight activation of both isozymes | Asterina amurensis | |
3.2.1.49 | Cu2+ | slight activation of both isozymes | Asterina amurensis | |
3.2.1.49 | K+ | slight activation of both isozymes | Asterina amurensis | |
3.2.1.49 | Mg2+ | slight activation of both isozymes | Asterina amurensis | |
3.2.1.49 | Na+ | slight activation of both isozymes | Asterina amurensis | |
3.2.1.49 | Zn2+ | slight activation of both isozymes | Asterina amurensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 40000 | - |
isozyme alpha-GalNAcase II, gel filtration | Asterina amurensis |
3.2.1.49 | 43000 | - |
1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE | Asterina amurensis |
3.2.1.49 | 46000 | - |
x * 46000, native enzyme, SDS-PAGE | Homo sapiens |
3.2.1.49 | 47000 | - |
x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE | Asterina amurensis |
3.2.1.49 | 430000 | - |
about, isozyme alpha-GalNAcase I, gel filtration | Asterina amurensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.49 | additional information | Homo sapiens | the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins | ? | - |
? | |
3.2.1.49 | additional information | Asterina amurensis | the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.49 | Asterina amurensis | - |
- |
- |
3.2.1.49 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.49 | native enzyme from placenta by 4-aminophenyl thio-beta-D-galactose-ceramide hexoside-resin affinity | Homo sapiens |
3.2.1.49 | native isozymes alpha-GalNAcase I and II are isolated from liver homogenate by ammonium sulfate fractionation and separated from each other by gel filtration. Then they are further individually purified by cation exchange chromatography and gel filtration, and additionally by hydroxylapatite chromatography for isozyme alpha-GalNAcase II. Isozyme alpha-GalNAcase I is purified 440fold, isozyme alpha-GalNAcase II 544fold | Asterina amurensis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.49 | alimentary canal | - |
Asterina amurensis | - |
3.2.1.49 | liver | - |
Asterina amurensis | - |
3.2.1.49 | placenta | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 4.5 | - |
purified placental enzyme | Homo sapiens |
3.2.1.49 | 19.56 | - |
purified isozyme alpha-GalNAcase I | Asterina amurensis |
3.2.1.49 | 24.2 | - |
purified isozyme alpha-GalNAcase II | Asterina amurensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.49 | GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O | i.e. Forssman hapten glycolipid | Homo sapiens | alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide | - |
? | |
3.2.1.49 | GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O | i.e. Forssman hapten glycolipid, production of globoside by both isozymes | Asterina amurensis | alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide | - |
? | |
3.2.1.49 | additional information | the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins | Homo sapiens | ? | - |
? | |
3.2.1.49 | additional information | the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins | Asterina amurensis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.49 | ? | x * 46000, native enzyme, SDS-PAGE | Homo sapiens |
3.2.1.49 | monomer | 1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE | Asterina amurensis |
3.2.1.49 | polymer | x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE | Asterina amurensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.49 | alpha-GalNAcase | - |
Homo sapiens |
3.2.1.49 | alpha-GalNAcase I | - |
Asterina amurensis |
3.2.1.49 | alpha-GalNAcase II | - |
Asterina amurensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 37 | - |
assay at | Homo sapiens |
3.2.1.49 | 37 | - |
assay at | Asterina amurensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 37 | - |
pH 4.0,m 60 min, purified isozyme alpha-GalNAcase I shows 50% remaining activity, purified isozyme alpha-GalNAcase II 92% | Asterina amurensis |
3.2.1.49 | 37 | - |
purified enzyme, 60 min, pH 4.0, 98% activity remaining | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 3 | - |
isozyme alpha-GalNAcase II | Asterina amurensis |
3.2.1.49 | 3.5 | - |
isozyme alpha-GalNAcase I | Asterina amurensis |
3.2.1.49 | 4.2 | - |
- |
Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.49 | 2.3 | 6.5 | purified isozyme alpha-GalNAcase II, stable at 4°C | Asterina amurensis |
3.2.1.49 | 2.7 | 4.5 | purified isozyme alpha-GalNAcase I, stable at 4°C | Asterina amurensis |
3.2.1.49 | 3.5 | 8.5 | purified enzyme, stable at 4°C | Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.49 | Asterina amurensis | purified isozyme alpha-GalNAcase I, isoelectric focusing | - |
4.4 |
3.2.1.49 | Homo sapiens | purified enzyme, isoelectric focusing | - |
4.8 |
3.2.1.49 | Asterina amurensis | purified isozyme alpha-GalNAcase II | - |
7.1 |