Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Harun-Or-Rashid, M.; Matsuzawa, T.; Satoh, Y.; Shiraishi, T.; Ando, M.; Sadik, G.; Uda, Y.
    Purification and characterization of alpha-N-acetylgalactosaminidases I and II from the starfish Asterina amurensis (2010), Biosci. Biotechnol. Biochem., 74, 256-261.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.49 Ag+ moderate inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II Asterina amurensis
3.2.1.49 Ag+
-
Homo sapiens
3.2.1.49 Ca2+
-
Homo sapiens
3.2.1.49 Cu2+
-
Homo sapiens
3.2.1.49 D-galactose about 10% inhibition of isozymes alpha-GalNAcase I and isozyme alpha-GalNAcase II Asterina amurensis
3.2.1.49 D-galactose
-
Homo sapiens
3.2.1.49 D-glucose
-
Homo sapiens
3.2.1.49 Hg2+ slight inhibition of isozyme alpha-GalNAcase I, strong inhibition of isozyme alpha-GalNAcase II Asterina amurensis
3.2.1.49 Hg2+
-
Homo sapiens
3.2.1.49 K+
-
Homo sapiens
3.2.1.49 Mg2+
-
Homo sapiens
3.2.1.49 Mn2+
-
Homo sapiens
3.2.1.49 additional information no inhibition by D-glucose, N-acetyl-D-glucosamine, and Mn2+ Asterina amurensis
3.2.1.49 N-acetyl-D-galactosamine 57% and 62% inhibition of isozyme alpha-GalNAcase I and isozyme alpha-GalNAcase II Asterina amurensis
3.2.1.49 N-acetyl-D-galactosamine
-
Homo sapiens
3.2.1.49 N-acetyl-D-glucosamine
-
Homo sapiens
3.2.1.49 Na+
-
Homo sapiens
3.2.1.49 Zn2+
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.49 1.04
-
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide pH 4.2, 37°C, isozyme alpha-GalNAcase I Asterina amurensis
3.2.1.49 1.79
-
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide pH 4.2, 37°C Homo sapiens
3.2.1.49 3.97
-
GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide pH 4.2, 37°C, isozyme alpha-GalNAcase II Asterina amurensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.49 Ca2+ slight activation of both isozymes Asterina amurensis
3.2.1.49 Cu2+ slight activation of both isozymes Asterina amurensis
3.2.1.49 K+ slight activation of both isozymes Asterina amurensis
3.2.1.49 Mg2+ slight activation of both isozymes Asterina amurensis
3.2.1.49 Na+ slight activation of both isozymes Asterina amurensis
3.2.1.49 Zn2+ slight activation of both isozymes Asterina amurensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.49 40000
-
isozyme alpha-GalNAcase II, gel filtration Asterina amurensis
3.2.1.49 43000
-
1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE Asterina amurensis
3.2.1.49 46000
-
x * 46000, native enzyme, SDS-PAGE Homo sapiens
3.2.1.49 47000
-
x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE Asterina amurensis
3.2.1.49 430000
-
about, isozyme alpha-GalNAcase I, gel filtration Asterina amurensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.49 additional information Homo sapiens the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins ?
-
?
3.2.1.49 additional information Asterina amurensis the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.49 Asterina amurensis
-
-
-
3.2.1.49 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.49 native enzyme from placenta by 4-aminophenyl thio-beta-D-galactose-ceramide hexoside-resin affinity Homo sapiens
3.2.1.49 native isozymes alpha-GalNAcase I and II are isolated from liver homogenate by ammonium sulfate fractionation and separated from each other by gel filtration. Then they are further individually purified by cation exchange chromatography and gel filtration, and additionally by hydroxylapatite chromatography for isozyme alpha-GalNAcase II. Isozyme alpha-GalNAcase I is purified 440fold, isozyme alpha-GalNAcase II 544fold Asterina amurensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.49 alimentary canal
-
Asterina amurensis
-
3.2.1.49 liver
-
Asterina amurensis
-
3.2.1.49 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.49 4.5
-
purified placental enzyme Homo sapiens
3.2.1.49 19.56
-
purified isozyme alpha-GalNAcase I Asterina amurensis
3.2.1.49 24.2
-
purified isozyme alpha-GalNAcase II Asterina amurensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.49 GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O i.e. Forssman hapten glycolipid Homo sapiens alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide
-
?
3.2.1.49 GalNAc-alpha-1,3-GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide + H2O i.e. Forssman hapten glycolipid, production of globoside by both isozymes Asterina amurensis alpha-N-acetylgalactosamine + GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-ceramide
-
?
3.2.1.49 additional information the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins Homo sapiens ?
-
?
3.2.1.49 additional information the enzyme hydrolyzes alpha-N-acetylgalactosyl residues from glycolipids and glycoproteins Asterina amurensis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.49 ? x * 46000, native enzyme, SDS-PAGE Homo sapiens
3.2.1.49 monomer 1 * 43000, isozyme alpha-GalNAcase II, SDS-PAGE Asterina amurensis
3.2.1.49 polymer x * 47000, isozyme alpha-GalNAcase I, SDS-PAGE Asterina amurensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.49 alpha-GalNAcase
-
Homo sapiens
3.2.1.49 alpha-GalNAcase I
-
Asterina amurensis
3.2.1.49 alpha-GalNAcase II
-
Asterina amurensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.49 37
-
assay at Homo sapiens
3.2.1.49 37
-
assay at Asterina amurensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.49 37
-
pH 4.0,m 60 min, purified isozyme alpha-GalNAcase I shows 50% remaining activity, purified isozyme alpha-GalNAcase II 92% Asterina amurensis
3.2.1.49 37
-
purified enzyme, 60 min, pH 4.0, 98% activity remaining Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.49 3
-
isozyme alpha-GalNAcase II Asterina amurensis
3.2.1.49 3.5
-
isozyme alpha-GalNAcase I Asterina amurensis
3.2.1.49 4.2
-
-
Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.49 2.3 6.5 purified isozyme alpha-GalNAcase II, stable at 4°C Asterina amurensis
3.2.1.49 2.7 4.5 purified isozyme alpha-GalNAcase I, stable at 4°C Asterina amurensis
3.2.1.49 3.5 8.5 purified enzyme, stable at 4°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.49 Asterina amurensis purified isozyme alpha-GalNAcase I, isoelectric focusing
-
4.4
3.2.1.49 Homo sapiens purified enzyme, isoelectric focusing
-
4.8
3.2.1.49 Asterina amurensis purified isozyme alpha-GalNAcase II
-
7.1