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Literature summary extracted from

  • Chen, W.M.; Chen, G.H.; Chen, C.S.; Jiang, S.T.
    Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system (2009), Biosci. Biotechnol. Biochem., 73, 1172-1174.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 2-mercaptoethanol
-
 Bacillus cereus
3.2.1.14 Urea
-
 Bacillus cereus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Co2+ activation Bacillus cereus
3.2.1.14 Mn2+ activation Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 69600
-
 x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme Bacillus cereus
3.2.1.14 74500
-
 x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus cereus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 proteolytic modification amino acid sequence lacks 33 N-terminal amino acids Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-D-GlcNAc + H2O 0.8% of the activity with 4-nitrophenyl-(beta-D-GlcNAc)3 Bacillus cereus 4-nitrophenol + N-acetyl-D-glucosamine
-
 ?
3.2.1.14 4-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Bacillus cereus 4-nitrophenol + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
 ?
3.2.1.14 carboxymethyl cellulose + H2O 1.9% of the activity with colloidal chitin Bacillus cereus ?
-
 ?
3.2.1.14 chitin powder + H2O 16.6% of the activity with colloidal chitin Bacillus cereus ?
-
 ?
3.2.1.14 chitosan + H2O 45.2% of the activity with colloidal chitin Bacillus cereus ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Bacillus cereus ?
-
 ?
3.2.1.14 glycol chitosan + H2O 3.0% of the activity with colloidal chitin Bacillus cereus ?
-
 ?
3.2.1.14 additional information no substrate: cellulose, Avicel PH-101 Bacillus cereus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme Bacillus cereus