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Literature summary extracted from

  • Nojiri, H.; Taira, H.; Iwata, K.; Morii, K.; Nam, J.W.; Yoshida, T.; Habe, H.; Nakamura, S.; Shimizu, K.; Yamane, H.; Omori, T.
    Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10 (2003), Biosci. Biotechnol. Biochem., 67, 36-45.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.13 expression in Escherichia coli Pseudomonas resinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.13 0.00251
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.13 31000
-
 2 * 31000, SDS-PAGe, 2 * 31300, calculated Pseudomonas resinovorans
3.7.1.13 31300
-
 2 * 31000, SDS-PAGe, 2 * 31300, calculated Pseudomonas resinovorans
3.7.1.13 70000
-
 gel filtration Pseudomonas resinovorans

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.13 Pseudomonas resinovorans Q9AQM4
-
-
3.7.1.13 Pseudomonas resinovorans CA10 Q9AQM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.13 recombinant enzyme Pseudomonas resinovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.13 3.84
-
 pH 7.5, 25°C Pseudomonas resinovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.13 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans anthranilate + 2-hydroxypenta-2,4-dienoate
-
 ?
3.7.1.13 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans CA10 anthranilate + 2-hydroxypenta-2,4-dienoate
-
 ?
3.7.1.13 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans ?
-
 ?
3.7.1.13 2-hydroxy-6-oxo-6-(2-hydroxyphenyl)hexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans CA10 ?
-
 ?
3.7.1.13 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans ?
-
 ?
3.7.1.13 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 Pseudomonas resinovorans CA10 ?
-
 ?
3.7.1.13 additional information hydrolytic activity decreases in the substrate order of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate, 2-hydroxy-6-oxo-6-(2'-hydroxyphenyl)hexa-2,4-dienoate. Poor substrates: 2-hydroxy-6-oxohepta-2,4-dienoic acid, 2-hydroxymuconic semialdehyde Pseudomonas resinovorans ?
-
 ?
3.7.1.13 additional information hydrolytic activity decreases in the substrate order of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate, 2-hydroxy-6-oxo-6-(2'-hydroxyphenyl)hexa-2,4-dienoate. Poor substrates: 2-hydroxy-6-oxohepta-2,4-dienoic acid, 2-hydroxymuconic semialdehyde Pseudomonas resinovorans CA10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.13 dimer 2 * 31000, SDS-PAGe, 2 * 31300, calculated Pseudomonas resinovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.13 55
-
 10 min, 75% residual activity Pseudomonas resinovorans
3.7.1.13 65
-
 10 min, inactivation Pseudomonas resinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.13 2.1
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate pH 7.5, 25°C Pseudomonas resinovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.13 7 7.5
-
 Pseudomonas resinovorans