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Literature summary extracted from

  • Li, A.N.; Yu, K.; Liu, H.Q.; Zhang, J.; Li, H.; Li, D.C.
    Two novel thermostable chitinase genes from thermophilic fungi: cloning, expression and characterization (2010), Biores. Technol., 101, 5546-5551.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Pichia pastoris Thermoascus aurantiacus
3.2.1.14 expression in Pichia pastoris Thermochaetoides thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 47300
-
 x * 47300, SDS-PAGE Thermochaetoides thermophila
3.2.1.14 48400
-
 x * 48400, SDS-PAGE Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Thermoascus aurantiacus A6YJX1 var. levisporus
-
3.2.1.14 Thermochaetoides thermophila B3GGC7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitosan + H2O 11.8% of the activity with colloidal chitin Thermoascus aurantiacus chitosan oligosaccharides
-
 ?
3.2.1.14 chitosan + H2O 17.4% of the activity with colloidal chitin Thermochaetoides thermophila chitosan oligosaccharides
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Thermoascus aurantiacus GlcNAcbeta(1-4)GlcNAc + ? major product ?
3.2.1.14 colloidal chitin + H2O
-
 Thermochaetoides thermophila GlcNAcbeta(1-4)GlcNAc + ? major product ?
3.2.1.14 additional information no substrate: 4-nitrophenyl-(GlcNAc) Thermoascus aurantiacus ?
-
 ?
3.2.1.14 additional information no substrate: 4-nitrophenyl-(GlcNAc) Thermochaetoides thermophila ?
-
 ?
3.2.1.14 powdery chitin + H2O 15.4% of the activity with colloidal chitin Thermoascus aurantiacus ?
-
 ?
3.2.1.14 powdery chitin + H2O 32.2% of the activity with colloidal chitin Thermochaetoides thermophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 47300, SDS-PAGE Thermochaetoides thermophila
3.2.1.14 ? x * 48400, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Thermoascus aurantiacus
3.2.1.14 60
-
-
 Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 60 min, 95% residual activity Thermoascus aurantiacus
3.2.1.14 60
-
 60 min, 96.7% residual activity Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5.5
-
-
 Thermochaetoides thermophila
3.2.1.14 8
-
-
 Thermoascus aurantiacus