BRENDA - Enzyme Database

Crystal structure of an S-formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125

Alterio, V.; Aurilia, V.; Romanelli, A.; Parracino, A.; Saviano, M.; D'Auria, S.; De Simone, G.; Biopolymers 93, 669-677 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.2.12
N-terminus His6-S-tagged protein from pET28a construct overexpressed in Escherichia coli strain BL21(DE3) under the control of the T7 RNA polymerase transcription system
Pseudoalteromonas haloplanktis
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.2.12
by the hanging drop vapor diffusion technique, to 2.2 A resolution. Crystals belong to space group P212121 with unit cell dimensions of a = 49.49 A, b = 129.75 A, c = 152.67 A, with 4 molecules per asymmetric unit
Pseudoalteromonas haloplanktis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
3.1.2.12
60000
-
gel filtration
Pseudoalteromonas haloplanktis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Pseudoalteromonas haloplanktis
-
TAC125
-
3.1.2.12
Pseudoalteromonas haloplanktis TAC 125
-
TAC125
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
to homogeneity by a single-step Ni2+ affinity chromatography
Pseudoalteromonas haloplanktis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis
4-nitrophenol + thioacetate
-
-
-
?
3.1.2.12
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis TAC 125
4-nitrophenol + thioacetate
-
-
-
?
3.1.2.12
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis
?
-
-
-
-
3.1.2.12
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis TAC 125
?
-
-
-
-
3.1.2.12
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis
glutathione + acetate
-
-
-
?
3.1.2.12
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + acetate
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis
glutathione + formate
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + formate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
N-terminus His6-S-tagged protein from pET28a construct overexpressed in Escherichia coli strain BL21(DE3) under the control of the T7 RNA polymerase transcription system
Pseudoalteromonas haloplanktis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.2.12
by the hanging drop vapor diffusion technique, to 2.2 A resolution. Crystals belong to space group P212121 with unit cell dimensions of a = 49.49 A, b = 129.75 A, c = 152.67 A, with 4 molecules per asymmetric unit
Pseudoalteromonas haloplanktis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis
3.1.2.12
60000
-
gel filtration
Pseudoalteromonas haloplanktis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
to homogeneity by a single-step Ni2+ affinity chromatography
Pseudoalteromonas haloplanktis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis
4-nitrophenol + thioacetate
-
-
-
?
3.1.2.12
4-nitrophenyl thioacetate + H2O
substrate with small acyl moiety
707759
Pseudoalteromonas haloplanktis TAC 125
4-nitrophenol + thioacetate
-
-
-
?
3.1.2.12
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis
?
-
-
-
-
3.1.2.12
additional information
PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold
707759
Pseudoalteromonas haloplanktis TAC 125
?
-
-
-
-
3.1.2.12
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis
glutathione + acetate
-
-
-
?
3.1.2.12
S-acetylglutathione + H2O
substrate with small acyl moiety, high activity
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + acetate
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis
glutathione + formate
-
-
-
?
3.1.2.12
S-formylglutathione + H2O
substrate with small acyl moiety, lower activity than compared to S-acetylglutathione
707759
Pseudoalteromonas haloplanktis TAC 125
glutathione + formate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 30000, SDS-PAGE
Pseudoalteromonas haloplanktis