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Literature summary extracted from

  • Arora, N.; Banerjee, A.K.; Mutyala, S.; Murty, U.S.
    Comparative characterization of commercially important xylanase enzymes (2009), Bioinformation, 3, 446-453.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.8 paper production since XynA is thermostable and has no cellulase activity it can be potentially used for pretreatment of paper pulp before bleaching Paecilomyces sp. 'thermophila'

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene xynA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Paecilomyces sp. 'thermophila'

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Michaelis-Menten kinetics, Km values of XynA for birchwood xylan, beechwood xylan, and oat-spelt xylan are 4.4 mg/ml, 3.6 mg/ml, and 9.7 mg/ml, respectively Paecilomyces sp. 'thermophila'

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 26132
-
 x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'
3.2.1.8 28000
-
 x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 additional information Paecilomyces sp. 'thermophila' beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone ?
-
 ?
3.2.1.8 additional information Paecilomyces sp. 'thermophila' J18 beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Paecilomyces sp. 'thermophila' D1G4K3 gene XynA
-
3.2.1.8 Paecilomyces sp. 'thermophila' J18 D1G4K3 gene XynA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein the protein sequence contains one possible N-glycosylation site, consensus Asn-Xaa-Ser/Thr, at position 25 and two potential O-glycosylation sites at positions 26/27, respectively Paecilomyces sp. 'thermophila'

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged enzyme 48fold from Escherichia coli by nickel affinity chromatography Paecilomyces sp. 'thermophila'

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 706.1
-
 purified recombinant XynA Paecilomyces sp. 'thermophila'

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beta-1,4-xylan + H2O xylan from birchwood, beechwood, and oat spelt Paecilomyces sp. 'thermophila' ?
-
 ?
3.2.1.8 beta-1,4-xylan + H2O xylan from birchwood, beechwood, and oat spelt Paecilomyces sp. 'thermophila' J18 ?
-
 ?
3.2.1.8 additional information beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone Paecilomyces sp. 'thermophila' ?
-
 ?
3.2.1.8 additional information the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation Paecilomyces sp. 'thermophila' ?
-
 ?
3.2.1.8 additional information beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone Paecilomyces sp. 'thermophila' J18 ?
-
 ?
3.2.1.8 additional information the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation Paecilomyces sp. 'thermophila' J18 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 beta-1,4endoxylanase
-
 Paecilomyces sp. 'thermophila'
3.2.1.8 xylanase
-
 Paecilomyces sp. 'thermophila'
3.2.1.8 XynA
-
 Paecilomyces sp. 'thermophila'

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
 recombinant His-tagged enzyme Paecilomyces sp. 'thermophila'

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 recombinant His-tagged enzyme, 30 min, over 90% activity remaining Paecilomyces sp. 'thermophila'
3.2.1.8 70
-
 recombinant His-tagged enzyme, half-life is 174.8 min Paecilomyces sp. 'thermophila'
3.2.1.8 75
-
 recombinant His-tagged enzyme, half-life is 137.9 min Paecilomyces sp. 'thermophila'
3.2.1.8 85
-
 recombinant His-tagged enzyme, 30 min, over 60% activity remaining, half-life is 68.2 min Paecilomyces sp. 'thermophila'

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7
-
 recombinant His-tagged enzyme Paecilomyces sp. 'thermophila'

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Paecilomyces sp. 'thermophila' sequence calculation
-
 5.47