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Literature summary extracted from

  • Li, N.; Wu, J.M.; Zhang, L.F.; Zhang, Y.Z.; Feng, H.
    Characterization of a unique proline iminopeptidase from white-rot basidiomycetes Phanerochaete chrysosporium (2010), Biochimie, 92, 779-788.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.5 expressed in Escherichia coli Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.5 D264A mutant shows no enzymatic activity Phanerodontia chrysosporium
3.4.11.5 H292L mutant shows no enzymatic activity Phanerodontia chrysosporium
3.4.11.5 S107D mutant shows no enzymatic activity Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 Al3+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 Ba2+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 benzamidine moderate inhibition Phanerodontia chrysosporium
3.4.11.5 Ca2+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 Co2+ strong inhibition Phanerodontia chrysosporium
3.4.11.5 Cu2+ almost inactivates the enzyme Phanerodontia chrysosporium
3.4.11.5 Fe2+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 Fe3+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 Hg2+ almost inactivates the enzyme Phanerodontia chrysosporium
3.4.11.5 hydrochloride moderate inhibition Phanerodontia chrysosporium
3.4.11.5 Mg2+ slight inhibition Phanerodontia chrysosporium
3.4.11.5 Mn2+ strong inhibition Phanerodontia chrysosporium
3.4.11.5 Ni2+ strong inhibition Phanerodontia chrysosporium
3.4.11.5 phenylmethanesulfonyl fluoride moderate inhibition Phanerodontia chrysosporium
3.4.11.5 Zn2+ almost inactivates the enzyme Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.5 2.15
-
 proline-4-nitroanilide Vmax: 347.86 micromol/min/mg Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 37620
-
 calculated from cDNA Phanerodontia chrysosporium
3.4.11.5 38000
-
 SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Phanerodontia chrysosporium C6KI04
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 L-proline 4-nitroanilide + H2O
-
 Phanerodontia chrysosporium L-proline + 4-nitroaniline
-
 ?
3.4.11.5 additional information the recombinant enzyme can cleave the peptides derived from enzyme-hydrolytic natural proteins to release free lysine Phanerodontia chrysosporium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.5 PchPiPA
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.5 45
-
 optimal temperature and assay at Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.5 218.1
-
 proline-4-nitroanilide
-
 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 8
-
 optimal pH and assay at Phanerodontia chrysosporium