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Literature summary extracted from

  • Sanchez, M.; Alvarez, M.A.; Balana, R.; Garrido-Pertierra, A.
    Properties and functions of two succinic-semialdehyde dehydrogenases from Pseudomonas putida (1988), Biochim. Biophys. Acta, 953, 249-257.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 2-mercaptoethanol
-
 Pseudomonas putida
1.2.1.16 dithiothreitol
-
 Pseudomonas putida
1.2.1.79 2-mercaptoethanol
-
 Pseudomonas putida
1.2.1.79 dithiothreitol
-
 Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 additional information not inhibitory: succinate, pyruvate Pseudomonas putida
1.2.1.16 NADPH
-
 Pseudomonas putida
1.2.1.79 additional information not inhibitory: succinate, pyruvate Pseudomonas putida
1.2.1.79 NADPH
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.0073
-
 succinate semialdehyde pH 8.0, 30°C Pseudomonas putida
1.2.1.16 0.107
-
 NAD+ pH 8.0, 30°C Pseudomonas putida
1.2.1.79 0.0199
-
 succinate semialdehyde pH 8.0, 30°C Pseudomonas putida
1.2.1.79 0.135
-
 NADP+ pH 8.0, 30°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 53000
-
 2 * 53000, SDS-PAGE Pseudomonas putida
1.2.1.16 100000
-
 gel filtration Pseudomonas putida
1.2.1.79 200000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Pseudomonas putida
-
-
-
1.2.1.79 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.16 succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H + H+ mechanism is sequential, consistent with rapid-equilibrium ordered bi-bi system and a steady-state ordered bi-bi system Pseudomonas putida
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 40.7
-
 pH 8.0, 30°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Pseudomonas putida succinate + NADH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O at 9% of the rate with NAD+ Pseudomonas putida succinate + NADPH + H+
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
 Pseudomonas putida succinate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 dimer 2 * 53000, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+
-
 Pseudomonas putida
1.2.1.16 NADP+ at 9% of the rate with NAD+ Pseudomonas putida
1.2.1.79 NADP+ specific for NADP+ Pseudomonas putida

Expression

EC Number Organism Comment Expression
1.2.1.16 Pseudomonas putida induction by growth on 4-hydroxyphenylacetate additional information
1.2.1.79 Pseudomonas putida induction is highly coordinated with putrescine:2-oxoglutarate transaminase, gamma-aminobutyraldehyde dehydrogenase and gamma-aminobutyrate:2-oxoglutarate transaminase activities additional information