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Literature summary extracted from

  • Taira, T.; Fujiwara, M.; Dennhart, N.; Hayashi, H.; Onaga, S.; Ohnuma, T.; Letzel, T.; Sakuda, S.; Fukamizo, T.
    Transglycosylation reaction catalyzed by a class V chitinase from cycad, Cycas revoluta: A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS (2010), Biochim. Biophys. Acta, 1804, 668-675.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.14 analysis transglycosylation reaction takes place under the conditions used for HPLC analysis, but not under the ESI-MS conditions Cycas revoluta

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 F166A decrease in specific activity to 5-10% of wild-type, the sugar residue affinity of +1 subsite is strongly reduced. No effect on IC50 value of inhibitor allosamidin Cycas revoluta
3.2.1.14 W197A decrease in specific activity to 5-10% of wild-type, the sugar residue affinity of +1 subsite is strongly reduced. No effect on IC50 value of inhibitor allosamidin Cycas revoluta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 allosamidin
-
 Cycas revoluta

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Cycas revoluta
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Cycas revoluta 2 GlcNACbeta(1-4)GlcNAcbeta some (GlcNAc)3 is produced through transglycosylation reaction ?
3.2.1.14 GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Cycas revoluta GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
-
 ?
3.2.1.14 GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Cycas revoluta GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta product distribution is (GlcNAc)3 >(GlcNAc)4 > (GlcNAc)2 for the wild type enzyme. Some (GlcNAc)5 is produced, without formation of GlcNAc, suggesting that it is not directly produced from the initial substrate (GlcNAc)6 but produced through transglycosylation reaction ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.14 0.00057
-
 wild-type, pH 5.2 Cycas revoluta allosamidin
3.2.1.14 0.0006
-
 mutant F166A, pH 5.2 Cycas revoluta allosamidin
3.2.1.14 0.00091
-
 mutant W197A, pH 5.2 Cycas revoluta allosamidin