EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.52 | calpastatin | - |
Rattus norvegicus | |
3.4.22.52 | PD150606 | - |
Rattus norvegicus | |
3.4.22.53 | EGTA | - |
Rattus norvegicus | |
3.4.22.53 | PD150606 | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.52 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.4.22.53 | cytosol | - |
Rattus norvegicus | 5829 | - |
3.4.22.53 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.52 | Ca2+ | micromolar (0.005-0.05 mM) Ca2+ concentrations are required for activation in vitro | Rattus norvegicus | |
3.4.22.53 | Ca2+ | the Ca2+ dependency of mitochondrial m-calpain is similar to that of cytosolic m-calpain, 1 mM Ca2+ activates. | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.52 | 30000 | - |
1 * 80000 + 1 * 30000, SDS-PAGE | Rattus norvegicus |
3.4.22.52 | 80000 | - |
1 * 80000 + 1 * 30000, SDS-PAGE | Rattus norvegicus |
3.4.22.53 | 79920 | - |
m-calpain large subunit, calculated from amino acid sequence | Rattus norvegicus |
3.4.22.53 | 80000 | - |
1 * 80000 + 1 * ?, SDS-PAGE | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.52 | apoptosis-inducing factor + H2O | Rattus norvegicus | - |
truncated apoptosis-inducing factor + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.52 | Rattus norvegicus | - |
- |
- |
3.4.22.53 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.53 | DEAE-Sepharose column chromatography | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.52 | erythrocyte | - |
Rattus norvegicus | - |
3.4.22.52 | liver | - |
Rattus norvegicus | - |
3.4.22.53 | erythrocyte | - |
Rattus norvegicus | - |
3.4.22.53 | liver | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.52 | apoptosis-inducing factor + H2O | - |
Rattus norvegicus | truncated apoptosis-inducing factor + ? | - |
? | |
3.4.22.53 | casein + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.4.22.53 | voltage-dependent anion channel + H2O | mitochondrial m-calpain truncates voltage-dependent anion channel in Ca2+-dependent manner | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.52 | heterodimer | 1 * 80000 + 1 * 30000, SDS-PAGE | Rattus norvegicus |
3.4.22.53 | heterodimer | 1 * 80000 + 1 * ?, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.52 | mu-calpain | - |
Rattus norvegicus |
3.4.22.53 | calpain 2 | - |
Rattus norvegicus |
3.4.22.53 | mitochondrial m-calpain | m-calpain large subunit | Rattus norvegicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.4.22.53 | physiological function | mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria by cleaving voltage-dependent anion channel | Rattus norvegicus |