BRENDA - Enzyme Database

Phosphatidate degradation: phosphatidate phosphatases (lipins) and lipid phosphate phosphatases

Brindley, D.N.; Pilquil, C.; Sariahmetoglu, M.; Reue, K.; Biochim. Biophys. Acta 1791, 956-961 (2009)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
depends on
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
3.1.3.4
Mus musculus
-
-
-
3.1.3.4
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
3T3-L1 cell
lipin-1 and lipin-2
Mus musculus
-
3.1.3.4
fibroblast
-
Rattus norvegicus
-
3.1.3.4
muscle
-
Homo sapiens
-
3.1.3.4
preadipocyte cell line
lipin-2
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Mus musculus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
depends on
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
3T3-L1 cell
lipin-1 and lipin-2
Mus musculus
-
3.1.3.4
fibroblast
-
Rattus norvegicus
-
3.1.3.4
muscle
-
Homo sapiens
-
3.1.3.4
preadipocyte cell line
lipin-2
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Mus musculus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Homo sapiens
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
diacylglycerol is the necessary precursor for the synthesis of triacylglycerols, phosphatidylcholine and phosphatidylethanolamine
707555
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
3.1.3.4
Mus musculus
in the 3T3-L1 cell line, lipin-2 protein levels decline dramatically as adipocyte differentiation proceeds to become virtually undetectable in mature adipocytes, when lipin-1 is expressed at high levels
down
3.1.3.4
Mus musculus
in the 3T3-L1 cell line, lipin-2 protein levels are highest in preadipocytes
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
lipin-1 deficiency in rare human patients, causes acute myoglobinuria in childhood, does not result in lipodystrophy in these individuals. Muscle sample from a patient with lipin-1 deficiency reveals elevated phosphatidate levels. Rare patients with lipin-2 deficiency have a complex phenotype known as Majeed syndrome, characterized by recurrent osteomyelitis, fever, and anemia
Homo sapiens
3.1.3.4
malfunction
lipin-1 deficiency causes lipodystrophy, neonatal fatty liver, peripheral neuropathy, insulin resistance, and increased susceptibility to atherosclerosis
Mus musculus
3.1.3.4
physiological function
lipin proteins serve an important role in regulating the balance of lipid intermediates, including phosphatidic acid and diacylglycerol, and maintenance of cellular lipid homeostasis
Homo sapiens
3.1.3.4
physiological function
key role for lipin-1 in adipocyte differentiation and lipid biosynthesis
Mus musculus
3.1.3.4
physiological function
activation of fibroblasts by lysophosphatidate causes a translocation of Mg2+-dependent PAP activity to the membrane fraction within 2 min when the production of phosphatidic acid and diacylglycerol is increased by lysophosphatidate and platelet-derived growth factor. Translocations of PAP1 activity probably results from the increased presence of phosphatidic acid in membranes
Rattus norvegicus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.4
malfunction
lipin-1 deficiency in rare human patients, causes acute myoglobinuria in childhood, does not result in lipodystrophy in these individuals. Muscle sample from a patient with lipin-1 deficiency reveals elevated phosphatidate levels. Rare patients with lipin-2 deficiency have a complex phenotype known as Majeed syndrome, characterized by recurrent osteomyelitis, fever, and anemia
Homo sapiens
3.1.3.4
malfunction
lipin-1 deficiency causes lipodystrophy, neonatal fatty liver, peripheral neuropathy, insulin resistance, and increased susceptibility to atherosclerosis
Mus musculus
3.1.3.4
physiological function
lipin proteins serve an important role in regulating the balance of lipid intermediates, including phosphatidic acid and diacylglycerol, and maintenance of cellular lipid homeostasis
Homo sapiens
3.1.3.4
physiological function
key role for lipin-1 in adipocyte differentiation and lipid biosynthesis
Mus musculus
3.1.3.4
physiological function
activation of fibroblasts by lysophosphatidate causes a translocation of Mg2+-dependent PAP activity to the membrane fraction within 2 min when the production of phosphatidic acid and diacylglycerol is increased by lysophosphatidate and platelet-derived growth factor. Translocations of PAP1 activity probably results from the increased presence of phosphatidic acid in membranes
Rattus norvegicus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.4
Mus musculus
in the 3T3-L1 cell line, lipin-2 protein levels decline dramatically as adipocyte differentiation proceeds to become virtually undetectable in mature adipocytes, when lipin-1 is expressed at high levels
down
3.1.3.4
Mus musculus
in the 3T3-L1 cell line, lipin-2 protein levels are highest in preadipocytes
up