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Literature summary extracted from
- Potential role of phospholipase D2 in increasing interleukin-2 production by T-lymphocytes through activation of mitogen-activated protein kinases ERK1/ERK2 (2008), Biochim. Biophys. Acta, 1781, 263-269.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | additional information | PLD2 activity is enhanced by PMA/ionomycin stimulation | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | stable expression of PLD2 in Jurkat cells. Overexpression of a dominant negative PLD2 attenuates the early and sustained increase in ERK1/2 phosphorylation induced by PLD2, overview. Phosphorylation of ERK1/2 promoted by PMA-ionomycin in transfected Jurkat cells is inhibited by 1-butanol | Homo sapiens |
| 3.1.4.4 | stable expression of PLD2 in T hybridoma 2B4 cell line. Overexpression of a dominant negative PLD2 attenuates the early and sustained increase in ERK1/2 phosphorylation induced by PLD2, overview | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | additional information | interleukin-2 production evoked by PMA/ionomycin stimulation or CD3/CD28 engagement is enhanced in the two T-cell lines overexpressing PLD2 | Mus musculus |
| 3.1.4.4 | additional information | interleukin-2 production evoked by PMA/ionomycin stimulation or CD3/CD28 engagement is enhanced in the two T-cell lines overexpressing PLD2. hPLD2 overexpression results in an increase of ERK1/2 phosphorylation by about 50% within 60 min | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | 1-butanol | 1-butanol inhibits PLD2 and is potent in cancelling ERK1/2 activation | Homo sapiens |  |
| 3.1.4.4 | 1-butanol | 1-butanol inhibits PLD2 and is potent in cancelling ERK1/2 activation | Mus musculus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | - |
Mus musculus | |
| 3.1.4.4 | Ca2+ | - |
Homo sapiens | |
| 3.1.4.4 | Mg2+ | - |
Mus musculus | |
| 3.1.4.4 | Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | Mus musculus | hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, PA, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via PA and DAG production | choline + phosphatidic acid | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | Homo sapiens | hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via phosphatidic acid and diacylglycerol production | choline + phosphatidic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Homo sapiens | - |
isozyme PLD2 | - |
| 3.1.4.4 | Mus musculus | - |
isozyme PLD2 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | JURKAT cell | PLD2 is the main isoform present in the Jurkat cell line | Homo sapiens | - |
| 3.1.4.4 | K-562 cell | a myeloid cell line | Homo sapiens | - |
| 3.1.4.4 | KARPAS-422 cell | a B lymphoid cell line | Homo sapiens | - |
| 3.1.4.4 | KG-1A cell | a myeloid cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | additional information | PLD assay on whole haematopoietic cells | Mus musculus | ? | - |
? | |
| 3.1.4.4 | additional information | PLD assay on whole hematopoietic cells | Homo sapiens | ? | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Mus musculus | choline + phosphatidate | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Homo sapiens | choline + phosphatidate | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, PA, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via PA and DAG production | Mus musculus | choline + phosphatidic acid | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via phosphatidic acid and diacylglycerol production | Homo sapiens | choline + phosphatidic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | phospholipase D2 | - |
Mus musculus |
| 3.1.4.4 | phospholipase D2 | - |
Homo sapiens |
| 3.1.4.4 | PLD | - |
Mus musculus |
| 3.1.4.4 | PLD | - |
Homo sapiens |
| 3.1.4.4 | PLD2 | - |
Mus musculus |
| 3.1.4.4 | PLD2 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 37 | - |
assay at | Mus musculus |
| 3.1.4.4 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 7.4 | - |
assay at | Mus musculus |
| 3.1.4.4 | 7.4 | - |
assay at | Homo sapiens |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.1.4.4 | Homo sapiens | an increase in PLD2 expression correlates with an enhanced ERK-1/-2 activation following PMA/ionomycin stimulation | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | metabolism | phosphatidic acid is a source of diacylglycerol, the two versatile lipid second messengers are at the centre of a phospholipid signalling network and as such are involved in several cellular functions | Mus musculus |
| 3.1.4.4 | metabolism | phosphatidic acid is a source of diacylglycerol, the two versatile lipid second messengers are at the centre of a phospholipid signalling network and as such are involved in several cellular functions | Homo sapiens |
| 3.1.4.4 | physiological function | PLD acting upstream of the MAP kinases ERK1/2 may play a key role in the regulation of IL-2 production by stimulated Jurkat cells. PLD2 promotes an early and sustained increase in ERK1/2 phosphorylation in recombinant T-cell lines, which is inhibited by 1-butanol | Homo sapiens |
| 3.1.4.4 | physiological function | PLD2 promotes an early and sustained increase in ERK1/2 phosphorylation in recombinant T-cell lines, which is inhibited by 1-butanol. | Mus musculus |
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