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Literature summary extracted from

  • Kuo, T.-H.; Liang, P.-H.
    Reaction kinetic pathway of the recombinant octaprenyl pyrophosphate synthase from Thermotoga maritima: how is it different from that of the mesophilic enzyme (2002), Biochim. Biophys. Acta, 1599, 125-133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.90
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.90 0.0015
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C Thermotoga maritima
2.5.1.90 0.002
-
 isopentenyl diphosphate pH 7.5, 25°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.90 Thermotoga maritima
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-octaprenyl diphosphate isopentenyl diphosphate condensation rather than product release is rate limiting Thermotoga maritima
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.90 (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate
-
 Thermotoga maritima 5 diphosphate + all-trans-octaprenyl diphosphate the product of chain elongation is C40 and the rate of its conversion to C45 is negligible. Under single-turnover condition with 0.01 mM enzyme-farnesyl diphosphate complex and 0.001 mM isopentenyl diphosphate, only the C20 is formed ?
2.5.1.90 additional information the isopentenyl diphosphate condensation represents the rate-limiting step of the reaction Thermotoga maritima ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.90 80
-
 melting temeprature is abve 80°C Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.90 0.005
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 25°C Thermotoga maritima