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Literature summary extracted from

  • Ignatov, K.B.; Kramarov, V.M.
    DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences (2009), Biochemistry, 74, 557-561.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 expressed in Escherichia coli Thermus aquaticus
6.5.1.2 expressed in Escherichia coli Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus aquaticus
-
 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus thermophilus
-
 AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Thermus aquaticus
-
-
-
6.5.1.2 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2 DEAE column chromatography and phosphor-cellulose column chromatography Thermus thermophilus
6.5.1.2 DEAE column chromatographyand phosphor-cellulose column chromatography Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermus aquaticus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermus thermophilus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 NAD+-dependent DNA ligase
-
 Thermus aquaticus
6.5.1.2 NAD+-dependent DNA ligase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ NAD+ presence is essential for exhibiting DNA ligase activity Thermus aquaticus
6.5.1.2 NAD+ NAD+ presence is essential for exhibiting DNA ligase activity Thermus thermophilus

General Information

EC Number General Information Comment Organism
6.5.1.2 physiological function DNA ligase enhances efficiency of DNA polymerase in long PCR amplification Thermus aquaticus
6.5.1.2 physiological function DNA ligase enhances efficiency of DNA polymerase in long PCR amplification Thermus thermophilus